SORBS1 Antibody

Images

 
Western Blot: SORBS1 Antibody [NBP1-86641] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Tonsil
Immunocytochemistry/ Immunofluorescence: SORBS1 Antibody [NBP1-86641] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, centrosome & focal adhesion sites.
Immunohistochemistry-Paraffin: SORBS1 Antibody [NBP1-86641] - Staining of human heart muscle shows strong membranous and cytoplasmic positivity in myocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SORBS1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:DSSPLLNEVSSSLIGTDSQAFPSVSKPSSAYPSTTIVNPTIVLLQHNREQQKRLSSLSDPVSERRVGEQDSAPTQEKPTSPGKAIEKRAKDDSRRVVKSTQDLSDVSMDEVGIPLRNTERSKDWYKTMFKQIHKLNR
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SORBS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunofluorescence
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SORBS1 Protein (NBP1-86641PEP)
Publications
Read Publication using NBP1-86641.

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (84%), Rat (85%). Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SORBS1 Antibody

  • c-Cbl associated protein
  • c-Cbl-associated protein
  • DKFZp451C066
  • DKFZp586P1422
  • Fas-ligand associated factor 2
  • FLAF2
  • KIAA0894
  • KIAA1296CAPSH3D5SH3P12FLJ12406
  • ponsin
  • R85FL
  • SH3 domain protein 5
  • SH3-domain protein 5 (ponsin)
  • sh3p12
  • SORB1
  • sorbin and SH3 domain containing 1
  • sorbin and SH3 domain-containing protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SORBS1 Antibody (NBP1-86641)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SORBS1