SOD2/Mn-SOD Antibody - BSA Free

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535] - Human breast carcinoma. SOD2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:1000.

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:1000.

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Rat tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:2500.

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

SOD2 antibody detects SOD2 protein by western blot analysis. Mouse tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (NBP2-20535) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

SOD2 antibody detects SOD2 protein by western blot analysis. Rat tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (NBP2-20535) diluted by 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (NBP2-20535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Al, Av
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SOD2/Mn-SOD Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SOD2/Mn-SOD Antibody - BSA Free (NBP2-20535) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-SOD2/Mn-SOD Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human SOD2/Mn-SOD Recombinant protein.
• Localization: Mitochondrion matrix
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOD2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen Assay dependent
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SOD2/Mn-SOD Antibody - BSA Free

• EC 1.15.1.1
• indophenoloxidase B
• IPOB
• IPO-B
• manganese-containing superoxide dismutase
• mangano-superoxide dismutase
• Mn SOD
• Mn superoxide dismutase
• MnSOD
• MVCD6
• SOD, Mitochodrial
• SOD2
• superoxide dismutase [Mn], mitochondrial
• superoxide dismutase 2, mitochondrial

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOD2/Mn-SOD Antibody (NBP2-20535)(7)

Publications using NBP2-20535

• Lv Y, Zhu D, Li J et al. Plant-derived hydrogel and photosynthetic nano-units for myocardial infarction therapy. Nature Communications 2025-08-18 [PMID: 40825772]
• Piotrowska K, Zgutka K, Tomasiak P et al. Every-other day (EOD) feeding regime decreases oxidative stress and inflammatory cascade in mouse liver: The immunohistochemical study Tissue & cell 2023-10-06 [PMID: 37812950] (Immunohistochemistry, Mouse)
• Hernandez J, Sch�ffer J, Herden C et al. n-3 Polyunsaturated Fatty Acids Modulate LPS-Induced ARDS and the Lung-Brain Axis of Communication in Wild-Type versus Fat-1 Mice Genetically Modified for Leukotriene B4 Receptor 1 or Chemerin Receptor 23 Knockout International Journal of Molecular Sciences 2023-08-31 [PMID: 37686333] (Western Blot, Rat)
• Gallo G, Forte M, Cotugno M et al. Polymorphic variants at NDUFC2, encoding a mitochondrial complex I subunit, associate with cardiac hypertrophy in human hypertension Molecular Medicine 2023-08-09 [PMID: 37558995] (Western Blot, Rat)
• Lee J, Jung SY, Yang KJ et al. alpha-Lipoic acid prevents against cisplatin cytotoxicity via activation of the NRF2/HO-1 antioxidant pathway PLoS ONE 2019-12-26 [PMID: 31877176] (WB, Mouse)
• Vidimar V, Gius D, Chakravarti D et al. Dysfunctional MnSOD leads to redox dysregulation and activation of prosurvival AKT signaling in uterine leiomyomas. Sci Adv. 2016-11-01 [PMID: 27847869] (IF/IHC, Human)
• Davis SM, Collier LA, Leonardo CC et al. Leukemia Inhibitory Factor Protects Neurons from Ischemic Damage via Upregulation of Superoxide Dismutase 3. Mol. Neurobiol. 2016-01-09 [PMID: 26746670] (WB, Rat)

Review for SOD2/Mn-SOD Antibody (NBP2-20535) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 05/06/2013

Summary

• Application: Western Blot
• Sample Tested: mouse liver lysate
• Species: Mouse

Bioinformatics

• Gene Symbol: SOD2