SOD2/Mn-SOD Antibody

Images

 
Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody. HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535] - Paraffin-embedded human breast carcinoma. SOD2 antibody diluted at 1:500.
Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:1000.
Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:1000.
Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Rat tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted by 1:2500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Order Details

SOD2/Mn-SOD Antibody Summary

Immunogen
Full length human SOD2 Recombinant protein.
Localization
Mitochondrion matrix
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SOD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-20535 in the following applications:

Publications
Read Publications using
NBP2-20535 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOD2/Mn-SOD Antibody

  • EC 1.15.1.1
  • indophenoloxidase B
  • IPOB
  • IPO-B
  • manganese-containing superoxide dismutase
  • mangano-superoxide dismutase
  • Mn SOD
  • Mn superoxide dismutase
  • MnSOD
  • MVCD6
  • SOD, Mitochodrial
  • SOD2
  • superoxide dismutase [Mn], mitochondrial
  • superoxide dismutase 2, mitochondrial

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOD2/Mn-SOD Antibody (NBP2-20535)(2)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(1)
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Human
(1)
Rat
(1)
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Review for SOD2/Mn-SOD Antibody (NBP2-20535) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-20535:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot SOD2/Mn-SOD NBP2-20535
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reviewed by:
Anonymous
WB Mouse 05/06/2013
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Summary

ApplicationWestern Blot
Sample Testedmouse liver lysate
SpeciesMouse
CommentsWe used Dounce homogenization and a mild lysis buffer (20 mM Tris pH 7.5, 1% Triton X-100, 0.5 mM EDTA) to make our mouse liver extracts. We electrophoresed 100 ug of mouse liver lysates on Life Technologies NuPage 10% Bis-Tris Gels using MES running buffer. We transferred the gels to ECL nitrocellulose membrane and then probed the blot with the antibody as recommended. Overall the antibody worked very well.

Blocking

Blocking Details5% non-fat skim milk in 1x TBS-T for 1 hour at room temperature with rocking

Primary Anitbody

Dilution Ratio1:1000, incubated in blocking buffer overnight at 4 degrees C with rocking

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP-conjugated
Secondary Manufacturer Cat#from ECL kit
Secondary Concentration1:5000

Details

Detection NotesECL; 10 second exposure

Comments

CommentsWe used Dounce homogenization and a mild lysis buffer (20 mM Tris pH 7.5, 1% Triton X-100, 0.5 mM EDTA) to make our mouse liver extracts. We electrophoresed 100 ug of mouse liver lysates on Life Technologies NuPage 10% Bis-Tris Gels using MES running buffer. We transferred the gels to ECL nitrocellulose membrane and then probed the blot with the antibody as recommended. Overall the antibody worked very well.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOD2/Mn-SOD Antibody (NBP2-20535) (0)

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Secondary Antibodies

 

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Recent Reviews

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Anonymous
05/06/2013
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SOD2
Entrez