SOD2/Mn-SOD Antibody

Images

 
Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NB100-1969] - analysis using Rabbit Anti-SOD2 Polyclonal Antibody. Tissue: muscle fibres. Species: Rat. Primary Antibody: Rabbit Anti-SOD2 Polyclonal Antibody at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SOD2/Mn-SOD Antibody Summary

Immunogen
Human Mn SOD.
Localization
Mitochondrion, Mitochondrion Matrix
Specificity
Detects approx 25kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SOD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
0.2 ug/ml of SOD (Mn) Antibody was sufficient for detection of Mn SOD in 20 ug of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-mouse IgG:AP as the secondary Antibody. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24578415).
Positive Control
Brain Lysate (NB820-59705)
Publications
Read Publications using
NB100-1969 in the following applications:

Reactivity Notes

Based on homology, It's predicted to detect Rat, Mouse, Bovine, Canine, Chicken, Gerbil, Guinea Pig, Porcine, Hamster, Monkey, Rabbit, Sheep, Xenopus, Frog and Wood Frog (Rana sylvatica).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOD2/Mn-SOD Antibody

  • EC 1.15.1.1
  • indophenoloxidase B
  • IPOB
  • IPO-B
  • manganese-containing superoxide dismutase
  • mangano-superoxide dismutase
  • Mn SOD
  • Mn superoxide dismutase
  • MnSOD
  • MVCD6
  • SOD, Mitochodrial
  • SOD2
  • superoxide dismutase [Mn], mitochondrial
  • superoxide dismutase 2, mitochondrial

Background

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(8). SOD3 can also be distinguished by its heparin-binding capacity (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOD2/Mn-SOD Antibody (NB100-1969)(5)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SOD2
Entrez
Uniprot