0.2 ug/ml of SOD (Mn) Antibody was sufficient for detection of Mn SOD in 20 ug of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-mouse IgG:AP as the secondary Antibody. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24578415).
Based on homology, It's predicted to detect Rat, Mouse, Bovine, Canine, Chicken, Gerbil, Guinea Pig, Porcine, Hamster, Monkey, Rabbit, Sheep, Xenopus, Frog and Wood Frog (Rana sylvatica).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.0) and 50% Glycerol
0.09% Sodium Azide
Immunogen affinity purified
Alternate Names for SOD2/Mn-SOD Antibody
manganese-containing superoxide dismutase
Mn superoxide dismutase
superoxide dismutase [Mn], mitochondrial
superoxide dismutase 2, mitochondrial
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(8). SOD3 can also be distinguished by its heparin-binding capacity (1).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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