Recombinant Human SOD1/Cu-Zn SOD Protein

SDS-Page: Human SOD1/Cu-Zn SOD Protein [NBP2-34940]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human SOD1/Cu-Zn SOD Protein Summary

• Description: A homodimer, non-glycosylated polypeptide chain containing two 153 amino acid chains corresponding to SOD1/Cu-Zn SOD Source: E. coli

  Uniprot ID: P00441

  Amino Acid Sequence: ATKAVCVLKG DGPVQGIINF EQKESNGPVK VWGSIKGLTE GLHGFHVHEF GDNTAGCTSA GPHFNPLSRK HGGPKDEERH VGDLGNVTAD KDGVADVSIE DSVISLSGDH CIIGRTLVVH EKADDLGKGG NEESTKTGNA GSRLACGVIG IAQ

• Details of Functionality: SOD1/Cu-Zn SOD protein is fully biologically active when compared to standard. The potency per mg was determined by pyrogallol autoxidation method and was found to be more than 3000 U/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SOD1
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of SOD1/Cu-Zn SOD as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 31.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human SOD1/Cu-Zn SOD Protein

• ALS
• ALS1
• amyotrophic lateral sclerosis 1 (adult)
• Cu
• Cu/Zn superoxide dismutase
• CuZn SOD
• Cu-Zn SOD
• EC 1.15.1.1
• homodimer
• hSod1
• indophenoloxidase A
• Ipo1
• IPOA
• SOD
• SOD, cytosolic
• SOD, Soluble
• SOD1
• superoxide dismutase [Cu-Zn]
• Superoxide dismutase 1
• superoxide dismutase 1, soluble
• Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

Background

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -70 degrees C. Avoid repeated freeze/thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SOD1
• Uniprot:
  • P00441()
  • P00441()
  • P00441()