Recombinant Human SOD1/Cu-Zn SOD Protein Summary
Description |
Recombinant bioactive protein for Human SOD1/Cu-Zn SOD
Source:E. coli Amino Acid Sequence:(P00441)
This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab112193) |
Preparation Method |
Determined to be >98% pure by SDS-PAGE |
Details of Functionality |
Recombinant Superoxide Dismutase is fully biologically active when compared to standard. Activity tests were carried using SOD activity assay kit. The activity assay kit showed that the calculated activity was ~40,000 U/mg. This was calculated from the amount (ug) required to inhibit the Xanthine Oxidase activity by 50% |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
SOD1 |
Purity |
>98%, by SDS-PAGE |
Endotoxin Note |
<0.1 ng/ug |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
16.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Buffer |
Lyophilized |
Concentration |
Lyoph |
Purity |
>98%, by SDS-PAGE |
Reconstitution Instructions |
Reconstitute in H2O to >100 g/ml. The solution can then be diluted into other aqueous buffers. |
Alternate Names for Recombinant Human SOD1/Cu-Zn SOD Protein
Background
Superoxide Dismutase (SOD) is an oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. Recombinant Human Cu/Zn Superoxide Dismutase produced in E.Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 154 amino acids and having a total molecular mass of 31,608 Dalton. The Cu/Zn SOD is purified by proprietary chromatographic techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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