SOD1/Cu-Zn SOD Antibody

Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-1973] - Western blot analysis of Human Cell line lysates showing detection of SOD1/Cu-Zn SOD protein using Rabbit Anti-SOD1/Cu-Zn SOD Polyclonal Antibody (NB100-1973). Load: 15 ugprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-SOD1/Cu-Zn SOD Polyclonal Antibody (NB100-1973) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Product Discontinued

SOD1/Cu-Zn SOD Antibody Summary

• Immunogen: Rat Cu/Zn SOD
• Localization: Cytoplasm
• Specificity: Detects approx 23kDa (human) and approx 19kDa (other species).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:120
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 0.5 ug/ml
• Application Notes: 0.5 ug/ml of SOD (Cu/Zn) Antibody was sufficient for detection of Cu/Zn SOD in 20 ug of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary Antibody.
• Control:

  SOD1/Cu-Zn SOD Overexpression Lysate
  HeLa Whole Cell Lysate

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Human, Mouse and Bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SOD1/Cu-Zn SOD Antibody

• ALS
• ALS1
• amyotrophic lateral sclerosis 1 (adult)
• Cu
• Cu/Zn superoxide dismutase
• CuZn SOD
• Cu-Zn SOD
• EC 1.15.1.1
• homodimer
• hSod1
• indophenoloxidase A
• Ipo1
• IPOA
• SOD
• SOD, cytosolic
• SOD, Soluble
• SOD1
• superoxide dismutase [Cu-Zn]
• Superoxide dismutase 1
• superoxide dismutase 1, soluble
• Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

Background

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin binding multimer of disulfide linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase(CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOD1
• Entrez:
  • 6647(Human)
  • 20655(Mouse)
  • 24786(Rat)