SOD1/Cu-Zn SOD Antibody

Western Blot: SOD1/Cu-Zn SOD Antibody [NB100-1955] - Western blot analysis of Human Cell line lysates showing detection of SOD1/Cu-Zn SOD protein using Rabbit Anti-SOD1/Cu-Zn SOD Polyclonal Antibody (NB100-1955). Load: 15 ugprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-SOD1/Cu-Zn SOD Polyclonal Antibody (NB100-1955) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Product Discontinued

SOD1/Cu-Zn SOD Antibody Summary

• Immunogen: Human Cu/Zn SOD
• Localization: Cytoplasm
• Specificity: Detects approx 23kDa (human) and approx 19kDa (other species).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 0.2 ug/ml
• Application Notes: 0.2 ug/ml of SOD (Cu/Zn) Antibody was sufficient for detection of Cu/Zn SOD in 20 ug of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary Antibody.
• Control:

  SOD1/Cu-Zn SOD Overexpression Lysate
  HeLa Whole Cell Lysate

Reactivity Notes

Based on homology, It is predicted to detect Rat , Mouse , Bovine , Monkey , Invertebrate , Coral (Anthozoa) , Canine , Hamster , Porcine , Rabbit , Sheep , Xenopus , Mollusk , Mussel (Perna viridis) , Fish , Insect and Butterfly (Ops melastigma)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0) and 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SOD1/Cu-Zn SOD Antibody

• ALS
• ALS1
• amyotrophic lateral sclerosis 1 (adult)
• Cu
• Cu/Zn superoxide dismutase
• CuZn SOD
• Cu-Zn SOD
• EC 1.15.1.1
• homodimer
• hSod1
• indophenoloxidase A
• Ipo1
• IPOA
• SOD
• SOD, cytosolic
• SOD, Soluble
• SOD1
• superoxide dismutase [Cu-Zn]
• Superoxide dismutase 1
• superoxide dismutase 1, soluble
• Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

Background

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2 to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(9). SOD3 can also be distinguished by its heparin-binding capacity (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOD1/Cu-Zn SOD Antibody (NB100-1955)(1)

Publication using NB100-1955

• Wojtera, E, Konior, A Et al. Obligatory role of intraluminal O2- in acute endothelin-1 and angiotensin II signaling to mediate endothelial dysfunction and MAPK activation in guinea-pig hearts. Int J Mol Sci 2014-10-27 [PMID: 25350109] (WB, Human)

Bioinformatics

• Gene Symbol: SOD1
• Entrez:
  • 6647(Human)
  • 20655(Mouse)
  • 24786(Rat)
• Uniprot:
  • NP_000445.1(Human)