SOCS-6 Antibody - BSA Free

Western Blot: SOCS-6 Antibody [NBP2-94197] - Analysis of extracts of various cell lines, using SOCS-6 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.

Immunohistochemistry-Paraffin: SOCS-6 Antibody [NBP2-94197] - Paraffin-embedded human mammary cancer using SOCS-6 .

Immunohistochemistry-Paraffin: SOCS-6 Antibody [NBP2-94197] - Paraffin-embedded human liver cancer using SOCS-6 .

Immunohistochemistry-Paraffin: SOCS-6 Antibody [NBP2-94197] - Paraffin-embedded human lung cancer using SOCS-6 .

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SOCS-6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SOCS-6 Antibody - BSA Free (NBP2-94197) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human SOCS6 (NP_004223.2). MKKISLKTLRKSFNLNKSKEETDFMVVQQPSLASDFGKDDSLFGSCYGKDMASCDINGEDEKGGKNRSKSESLMGTLKRRLSAKQKSKGKAGTPSGSSADEDTFSSSSAPIVFKDVRAQRPIRSTSLRSHHYSPAPWPLRPTNSEETCIKMEVRVKALVHSSSPSPALNGVRKDFHDLQSETTCQEQANSLKSSASHNGDLHLHLDEHVP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOCS6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SOCS-6 Antibody - BSA Free

• CIS4CIS-4
• Cytokine-inducible SH2 protein 4
• HSPC060
• SOCS4Cish4
• SOCS6
• SOCS-6
• SSI4SOCS-6
• STAI4
• STAT induced STAT inhibitor-4
• STAT4
• STATI4
• Suppressor of cytokine signaling 4SOCS-4
• suppressor of cytokine signaling 6

Background

SOCS6 is encoded by this gene contains a SH2 domain and a CIS homolog domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by GM-CSF and EPO in hematopoietic cells. A high expression level of this gene was found in factor-independent chronic myelogenous leukemia (CML) and erythroleukemia (HEL) cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOCS6