SOCS-5 Antibody

Western Blot: SOCS-5 Antibody [NB100-56639] - Analysis for SOCS5 using NB100-56639 at 1:500 dilution against 15 ug/lane of mouse spleen (lane A) and human spleen (lane B) lysates.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SOCS-5 Antibody Summary

• Description: Novus Biologicals Rabbit SOCS-5 Antibody (NB100-56639) is a polyclonal antibody validated for use in WB. Anti-SOCS-5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This polyclonal antibody was raised against a synthetic peptide containing amino acid residues of human SOCS5 protein. Human or mouse spleen lysate can be used as a positive control.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOCS5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Control:

  Human Spleen Whole Tissue Lysate (Adult Whole Normal)
  Mouse Spleen Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 19138703).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.1 mg/ml BSA.
• Preservative: 0.02% Sodium Azide
• Purity: Protein G purified

Alternate Names for SOCS-5 Antibody

• CIS6
• CIS6Cytokine-inducible SH2 protein 6
• CISH5
• CISH6
• CISH6CIS-6
• KIAA0671Cytokine-inducible SH2-containing protein 5
• SOCS5
• SOCS-5
• SOCS-5CISH5
• suppressor of cytokine signaling 5

Background

The suppressor of cytokine signaling (SOCS) family of proteins (SOCS1-SOCS7 and CIS) inhibit cytokine production by a classical negative feedback mechanism. These proteins are upregulated in response to cytokine stimulation followed by inhibition of cytokine-inducing signal pathway. Role of each member of the family has been determined by gene knockout experiments (1). SOCS5 protein is preferentially expressed in committed T-helper (Th1) cells. The action of SOCS5 is probably mediated by its interaction with the cytoplasmic domain of IL-4 receptor. During Th1 differentiation, there is a reduction in the association of Janus kinase 1 with the IL-4 receptor (IL-4R), which is correlated with the expression of SOCS5 pressor of cytokine signaling-5 (SOCS5). It has been also shown that in the overexpression of SOCS5 in transgenic mice leads to impaired Th2 development, suggesting that SOCS5 may plan an important role in the Th1 differentiation by regulating a balance between Th1 and Th2 (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOCS-5 Antibody (NB100-56639)(1)

Publication using NB100-56639

• Kuhad A, Bishnoi M, Tiwari V et al. Suppression of NF-kappabeta signaling pathway by tocotrienol can prevent diabetes associated cognitive deficits. Pharmacol Biochem Behav. 2009-04-01 [PMID: 19138703] (Rat)
  
  Details:
  Nuclear extract from rat cerebral cortex and hippocampus (Data not shown).

Bioinformatics

• Gene Symbol: SOCS5
• Entrez:
  • 56468(Mouse)
• Uniprot:
  • O75159()