SOCS-7/Nck/NAP4 Antibody (519106)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human SOCS‑7/Nck/NAP4 Monoclonal Antibody (Catalog # MAB5809) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS‑7/Nck/NAP4 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 519106
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SOCS-7/Nck/NAP4 Antibody (519106) Summary

• Immunogen: E. coli-derived recombinant human SOCS-7
  His351-Thr581
  Accession # O14512
• Specificity: Detects endogenous human SOCS-7 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOCS7
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOCS-7/Nck/NAP4 Antibody (519106)

• NAP4
• NAP-4
• NAP4SOCS-7
• Nck
• Nck, Ash and phospholipase C binding protein
• Nck, Ash and phospholipase C gamma-binding protein
• NCKAP4
• Nck-associated protein 4
• SOCS6
• SOCS7
• SOCS-7
• suppressor of cytokine signaling 7

Background

Suppressor of cytokine signaling 7 (SOCS-7; also Nck, Ash, and phospholipase C gamma-binding protein and Nck-associated protein 4) is a 63 kDa member of the SOCS family of proteins. It regulates signaling cascades through protein ubiquitination and/or sequestration. Human SOCS-7 is 581 amino acids (aa) in length and contains four poly-Pro regions, a poly-Gly region, a poly-Gln region, an SH2 domain, and a SOCS box domain. A second isoform for SOCS-7 has a deletion of aa 1‑250 found in isoform 1. Human SOCS-7 is 97% aa identical to mouse SOCS-7. SOCS-7 is constitutively expressed in several tissues, most strongly in the testis and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOCS7
• Uniprot:
  • O14512()