SMYD3 Antibody (2P8Z2)

Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102] - Western blot analysis of extracts of various cell lines, using SMYD3 Rabbit mAb (NBP3-16102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry/Immunofluorescence: SMYD3 Antibody (2P8Z2) [NBP3-16102] - Immunofluorescence analysis of NIH-3T3 cells using SMYD3 Rabbit mAb (NBP3-16102) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102] - Western blot analysis of extracts of various cell lines, using SMYD3 Rabbit mAb (NBP3-16102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody (2P8Z2) [NBP3-16102] - Confocal imaging of NIH/3T3 cells using SMYD3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 2P8Z2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SMYD3 Antibody (2P8Z2) Summary

• Description: Novus Biologicals Rabbit SMYD3 Antibody (2P8Z2) (NBP3-16102) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SMYD3 (Q9H7B4). SISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLMTSEERRKQLRDQYCFECDCFRCQTQDKDADMLTGDEQVWKEVQESLKKIEELKAHW
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMYD3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SMYD3 Antibody (2P8Z2)

• bA74P14.1 (novel protein)
• bA74P14.1
• EC 2.1.1
• EC 2.1.1.43
• FLJ21080
• KMT3E
• MGC104324
• MYND domain containing 1
• SET and MYND domain containing 3
• SET and MYND domain-containing protein 3
• SMYD3
• Zinc finger MYND domain-containing protein 1
• zinc finger protein, subfamily 3A (MYND domain containing), 1
• ZMYND1
• ZNFN3A1

Background

SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMYD3