Western Blot: Smad3 [p Thr179] Antibody [NBP2-19126] - NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or ...read more
ELISA: Smad3 [p Thr179] Antibody [NBP2-19126] - The SMAD pathway follows the canonical TGF- Betasignaling pathway. TGF- Betadimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and ...read more
Anti-Smad3 pT179 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue Thr179.
This antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. NBP2-19126 was cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad2. Reactivity against other phosphorylated Smad family members is not known.
Immunogen affinity purified
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Tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kDa in size corresponding to human phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Use in Immunohistochemistry paraffin reported in scientific literature (PMID 28706886).
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