Smad3 [p Thr179] Antibody

Images

 
Western Blot: Smad3 [p Thr179] Antibody [NBP2-19126] - NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or ...read more
ELISA: Smad3 [p Thr179] Antibody [NBP2-19126] - The SMAD pathway follows the canonical TGF- Betasignaling pathway. TGF- Betadimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Smad3 [p Thr179] Antibody Summary

Immunogen
Synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179. Protein accession number NP_005893.
Modification
p Thr179
Specificity
This antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. NBP2-19126 was cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad2. Reactivity against other phosphorylated Smad family members is not known.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMAD3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:15000-1:75000
  • Immunohistochemistry-Paraffin
Application Notes
Tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kDa in size corresponding to human phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Use in Immunohistochemistry paraffin reported in scientific literature (PMID 28706886).
Publications
Read Publication using
NBP2-19126 in the following applications:

Reactivity Notes

Reactive in Human. A BLAST analysis was used to suggest cross reactivity with Smad3 from Mouse, Rat, Porcine, Canine and Marmoset based on 100% sequence identity with the immunogen.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Smad3 [p Thr179] Antibody

  • DKFZp586N0721
  • DKFZp686J10186
  • hMAD-3
  • hSMAD3
  • HsT17436
  • JV15-2MGC60396
  • MAD homolog 3
  • mad protein homolog
  • MAD, mothers against decapentaplegic homolog 3 (Drosophila)
  • MAD, mothers against decapentaplegic homolog 3
  • mad3
  • MADH3mad homolog JV15-2
  • mothers against decapentaplegic homolog 3
  • Mothers against DPP homolog 3
  • pSMAD
  • SMA- and MAD-related protein 3
  • SMAD 3
  • SMAD family member 3HSPC193
  • SMAD, mothers against DPP homolog 3 (Drosophila)
  • SMAD, mothers against DPP homolog 3
  • Smad3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smad3 Antibody (NBP2-19126)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Human
(1)
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Video Protocols

WB Video Protocol

FAQs for Smad3 Antibody (NBP2-19126). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
    • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

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Bioinformatics

Gene Symbol SMAD3
Entrez
Uniprot