SLIRP Antibody - BSA Free

Western Blot: SLIRP Antibody [NB110-37258] - Detection of SLIRP protein in a human liver. Primary was used at 0.6 ug/ml.

Immunocytochemistry/Immunofluorescence: SLIRP Antibody [NB110-37258] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SLIRP (NB110-37258) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: SLIRP Antibody [NB110-37258] - Analysis of SLIRP in mouse intestine using DAB with hematoxylin counterstain.

Immunocytochemistry/Immunofluorescence: SLIRP Antibody [NB110-37258] - SLIRP antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Simple Western: SLIRP Antibody [NB110-37258] - Simple Western lane view shows a specific band for SLIRP in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

MUP1 is regulated by high-fat diet (HFD) and exercise training (ET). Heatmaps (A) of proteins altered (q < 0.05 and fold-change +/−50%) by ET on Chow (top), HFD (middle), or ET on HFD (bottom heatmap) with corresponding proteins from the other groups. Proteins depicted in (B) were either altered in the same direction by ET and ET on HFD, or they were oppositely altered by HFD and ET on Chow or ET on HFD (‘+’ denotes increased, ‘−’ decreased abundance; a diagonal strikethrough means that the protein was not significantly regulated). Quantification and representative images of western blot performed for the indicated proteins (C–G, n = 5–10). The effects of ET and HFD on Mup1 gene expression in muscle (H, n = 7–10), as well as the effect of ET effect on Mup1 gene expression in AMPK KD mice (I, n = 8). Data are means +/− SEM. ***p < 0.001 main effect of diet (HFD vs Chow). ###p < 0.001 and ##p < 0.01 main effects for activity (ET vs sedentary (SED)). ¤¤p < 0.01 difference between indicated groups. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30013070), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Pm
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SLIRP Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to a C-terminal region of human SLIRP protein. [Entrez-Prot# AAX58600]
• Localization: Cytoplasm, Mitochondria.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLIRP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 10 ug/ml
  • Western Blot 0.5-2.0 ug/ml
• Application Notes: In Western Blot, a band is seen approx. 12 kDa representing the SLIRP protein. May see additional bands above 45 kDa. In ICC/IF, staining of the mitochondria was observed in U2OS cells. In IHC-P, cytoplasmic and mitochondrial staining was observed in mouse intestine tissue.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Liver lysate 0.2 mg/mL, separated by Size, antibody dilution of 10 ug/mL. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  SLIRP Overexpression Lysate

• Control Peptide:

  SLIRP Peptide (NB110-37258PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SLIRP Antibody - BSA Free

• chromosome 14 open reading frame 156
• DC50
• PD04872
• SLIRP
• SRA stem-loop-interacting RNA-binding protein, mitochondrial

Background

SLIRP (SRA stem-loop-interacting RNA-binding protein) is RNA binding protein found predominantly in mitochondria and show some nuclear expression also where it is recruited to nuclear receptor target promoters. It is ubiquitously expressed with highest expression levels in heart, liver, skeletal muscle, testis etc. and has been reported in cancers also. SLIRP was initially identified in a yeast three-hybrid screen via its binding to STR7 loop of SRA RNA (stem-loop structure 7 of steroid receptor RNA activator), RNA activator that mediates activities of estrogen receptor. Inside the nucleus, SLIRP is recruited to estrogen-responsive promoters for repressing SRA-mediated coactivation. However, it is localized mainly in mitochondria, and disruption of mitochondrial localization sequence at its N-terminus diminishes its ability to repress transcription inside nucleus, suggesting that mitochondrial localization of SLIRP is crucial to its nuclear corepressor activity. Inside mitochondira, SLIRP is present in a large ribonucleoprotein complex and maintains mitochondrial mRNA homeostasis by regulating mRNA decay as well as polyadenylation. SLIRP also represses AR, TR (thyroid), GR (glucocorticoid) and VDR-mediated transactivation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLIRP Antibody (NB110-37258)(1)

Publication using NB110-37258

• kleinert M, Parker BL, Jensen TE et al. Quantitative proteomic characterization of cellular pathways associated with altered insulin sensitivity in skeletal muscle following high-fat diet feeding and exercise training. Sci Rep. 2018-07-16 [PMID: 30013070] (WB, Mouse)

Bioinformatics

• Gene Symbol: SLIRP