Western Blot: SLIRP Antibody [NB110-37258] - Detection of SLIRP protein in a human liver. Primary was used at 0.6 ug/ml.
Immunocytochemistry/ Immunofluorescence: SLIRP Antibody [NB110-37258] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry: SLIRP Antibody [NB110-37258] - Analysis of SLIRP in mouse intestine using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: SLIRP Antibody [NB110-37258] - SLIRP antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Simple Western: SLIRP Antibody [NB110-37258] - Simple Western lane view shows a specific band for SLIRP in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
This SLIRP antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen approx. 12 kDa representing the SLIRP protein. May see additional bands above 45 kDa. In ICC/IF, staining of the mitochondria was observed in U2OS cells. In IHC-P, cytoplasmic and mitochondrial staining was observed in mouse intestine tisSue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
12 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SLIRP (SRA stem-loop-interacting RNA-binding protein) is RNA binding protein found predominantly in mitochondria and show some nuclear expression also where it is recruited to nuclear receptor target promoters. It is ubiquitously expressed with highest expression levels in heart, liver, skeletal muscle, testis etc. and has been reported in cancers also. SLIRP was initially identified in a yeast three-hybrid screen via its binding to STR7 loop of SRA RNA (stem-loop structure 7 of steroid receptor RNA activator), RNA activator that mediates activities of estrogen receptor. Inside the nucleus, SLIRP is recruited to estrogen-responsive promoters for repressing SRA-mediated coactivation. However, it is localized mainly in mitochondria, and disruption of mitochondrial localization sequence at its N-terminus diminishes its ability to repress transcription inside nucleus, suggesting that mitochondrial localization of SLIRP is crucial to its nuclear corepressor activity. Inside mitochondira, SLIRP is present in a large ribonucleoprotein complex and maintains mitochondrial mRNA homeostasis by regulating mRNA decay as well as polyadenylation. SLIRP also represses AR, TR (thyroid), GR (glucocorticoid) and VDR-mediated transactivation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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