SLIRP Antibody

Images

 
Western Blot: SLIRP Antibody [NB110-37258] - Detection of SLIRP protein in a human liver. Primary was used at 0.6 ug/ml.
Immunocytochemistry/ Immunofluorescence: SLIRP Antibody [NB110-37258] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry: SLIRP Antibody [NB110-37258] - Analysis of SLIRP in mouse intestine using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: SLIRP Antibody [NB110-37258] - SLIRP antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Simple Western: SLIRP Antibody [NB110-37258] - Simple Western lane view shows a specific band for SLIRP in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

SLIRP Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region of human SLIRP protein. [Entrez-Prot# AAX58600]
Localization
Cytoplasm, Mitochondria.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLIRP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2.0 ug/ml
  • Simple Western 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This SLIRP antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen approx. 12 kDa representing the SLIRP protein. May see additional bands above 45 kDa. In ICC/IF, staining of the mitochondria was observed in U2OS cells. In IHC-P, cytoplasmic and mitochondrial staining was observed in mouse intestine tisSue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
SLIRP Overexpression Lysate
SLIRP Knockout 293T Cell Lysate
Control Peptide
SLIRP Peptide (NB110-37258PEP)
Publications
Read Publication using
NB110-37258 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SLIRP Antibody

  • chromosome 14 open reading frame 156
  • DC50
  • PD04872
  • SLIRP
  • SRA stem-loop-interacting RNA-binding protein, mitochondrial

Background

SLIRP (SRA stem-loop-interacting RNA-binding protein) is RNA binding protein found predominantly in mitochondria and show some nuclear expression also where it is recruited to nuclear receptor target promoters. It is ubiquitously expressed with highest expression levels in heart, liver, skeletal muscle, testis etc. and has been reported in cancers also. SLIRP was initially identified in a yeast three-hybrid screen via its binding to STR7 loop of SRA RNA (stem-loop structure 7 of steroid receptor RNA activator), RNA activator that mediates activities of estrogen receptor. Inside the nucleus, SLIRP is recruited to estrogen-responsive promoters for repressing SRA-mediated coactivation. However, it is localized mainly in mitochondria, and disruption of mitochondrial localization sequence at its N-terminus diminishes its ability to repress transcription inside nucleus, suggesting that mitochondrial localization of SLIRP is crucial to its nuclear corepressor activity. Inside mitochondira, SLIRP is present in a large ribonucleoprotein complex and maintains mitochondrial mRNA homeostasis by regulating mRNA decay as well as polyadenylation. SLIRP also represses AR, TR (thyroid), GR (glucocorticoid) and VDR-mediated transactivation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-37258
Species: Hu, Mu, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for SLIRP Antibody (NB110-37258)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLIRP Antibody (NB110-37258) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-37258AF350
Alexa Fluor 405 NB110-37258AF405
Alexa Fluor 488 NB110-37258AF488
Alexa Fluor 532 NB110-37258AF532
Alexa Fluor 594 NB110-37258AF594
Alexa Fluor 647 NB110-37258AF647
Alexa Fluor 700 NB110-37258AF700
Alexa Fluor 750 NB110-37258AF750
Biotin NB110-37258B
DyLight 350 NB110-37258UV
DyLight 405 NB110-37258V
DyLight 488 NB110-37258G
DyLight 550 NB110-37258R
DyLight 594 NB110-37258DL594
DyLight 650 NB110-37258C
DyLight 680 NB110-37258FR
DyLight 755 NB110-37258IR
FITC NB110-37258F
HRP NB110-37258H
Janelia Fluor 549 NB110-37258JF549
Janelia Fluor 646 NB110-37258JF646

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Bioinformatics

Gene Symbol SLIRP