SLIRP Antibody [FITC]

• Reactivity: Hu, Mu, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

SLIRP Antibody [FITC] Summary

• Immunogen: A synthetic peptide made to a C-terminal region of human SLIRP protein. [Entrez-Prot# AAX58600]
• Localization: Cytoplasm, Mitochondria.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLIRP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SLIRP Antibody [FITC]

• chromosome 14 open reading frame 156
• DC50
• PD04872
• SLIRP
• SRA stem-loop-interacting RNA-binding protein, mitochondrial

Background

SLIRP (SRA stem-loop-interacting RNA-binding protein) is RNA binding protein found predominantly in mitochondria and show some nuclear expression also where it is recruited to nuclear receptor target promoters. It is ubiquitously expressed with highest expression levels in heart, liver, skeletal muscle, testis etc. and has been reported in cancers also. SLIRP was initially identified in a yeast three-hybrid screen via its binding to STR7 loop of SRA RNA (stem-loop structure 7 of steroid receptor RNA activator), RNA activator that mediates activities of estrogen receptor. Inside the nucleus, SLIRP is recruited to estrogen-responsive promoters for repressing SRA-mediated coactivation. However, it is localized mainly in mitochondria, and disruption of mitochondrial localization sequence at its N-terminus diminishes its ability to repress transcription inside nucleus, suggesting that mitochondrial localization of SLIRP is crucial to its nuclear corepressor activity. Inside mitochondira, SLIRP is present in a large ribonucleoprotein complex and maintains mitochondrial mRNA homeostasis by regulating mRNA decay as well as polyadenylation. SLIRP also represses AR, TR (thyroid), GR (glucocorticoid) and VDR-mediated transactivation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLIRP