SPEN Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: SPEN Antibody [NBP1-82952] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: SPEN Antibody [NBP1-82952] - Staining of human rectum shows strong nuclear and slightly weaker cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SPEN Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:NRERAYEHSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYASRSRSPNRFDAHDPRYEPRAREQFTLPSVVHRDIYRDDITREVRGRRPERNYQHSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRSRSSSSDSI
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPEN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. Use in Immunoprecipitation reported in scientific literature (PMID 25915022)
Control Peptide
SPEN Protein (NBP1-82952PEP)
Publications
Read Publication using
NBP1-82952 in the following applications:

  • IP
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25915022).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SPEN Antibody

  • HIAA0929
  • KIAA0929nuclear receptor transcription cofactor
  • MINTMsx2 interacting nuclear target (MINT) homolog
  • RBM15C
  • SHARPmsx2-interacting protein
  • SMART/HDAC1 associated repressor protein
  • SMART/HDAC1-associated repressor protein
  • SPEN homolog
  • spen homolog, transcriptional regulator (Drosophila)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPEN Antibody (NBP1-82952)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IP.


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IP
(1)
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Mouse
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for SPEN Antibody (NBP1-82952). (Showing 1 - 1 of 1 FAQ).

  1. What is the concentration of your SPEN antibody (product NBP1-82952) with lot A81924?
    • Lot number A81924 of our SPEN antibody, NBP1-82952, is at a concentration of 0.3mg/ml.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SPEN Antibody (NBP1-82952)

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Calculators

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Bioinformatics

Gene Symbol SPEN