SPEN Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SPEN Antibody [NBP1-82952] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SPEN Antibody [NBP1-82952] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: SPEN Antibody [NBP1-82952] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.

Immunohistochemistry-Paraffin: SPEN Antibody [NBP1-82952] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: SPEN Antibody [NBP1-82952] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SPEN Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SPEN Antibody - BSA Free (NBP1-82952) is a polyclonal antibody validated for use in IHC, ICC/IF and IP. Anti-SPEN Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NRERAYEHSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYASRSRSPNRFDAHDPRYEPRAREQFTLPSVVHRDIYRDDITREVRGRRPERNYQHSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRSRSSSSDSI
• Predicted Species: Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPEN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation Reported in scientific literature (PMID 25915022)
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SPEN Protein (NBP1-82952PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25915022).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SPEN Antibody - BSA Free

• HIAA0929
• KIAA0929nuclear receptor transcription cofactor
• MINTMsx2 interacting nuclear target (MINT) homolog
• RBM15C
• SHARPmsx2-interacting protein
• SMART/HDAC1 associated repressor protein
• SMART/HDAC1-associated repressor protein
• SPEN homolog
• spen homolog, transcriptional regulator (Drosophila)

Background

SPEN encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPEN Antibody (NBP1-82952)(1)

Publication using NBP1-82952

• McHugh CA, Chen CK, Chow A et al. The Xist lncRNA interacts directly with SHARP to silence transcription through HDAC3 Nature 2015-04-27 [PMID: 25915022] (IP, Mouse)

FAQs for SPEN Antibody (NBP1-82952). (Showing 1 - 1 of 1 FAQ).

1. What is the concentration of your SPEN antibody (product NBP1-82952) with lot A81924?
   • Lot number A81924 of our SPEN antibody, NBP1-82952, is at a concentration of 0.3mg/ml.

Bioinformatics

• Gene Symbol: SPEN