PLEKHM2 Antibody

Images

 
Western Blot: PLEKHM2 Antibody [NBP1-77089] - Rat brain tissue lysate with PLEKHM2 antibody at 0.5 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: PLEKHM2 Antibody [NBP1-77089] - Staining of Human Brain cells at 20ug/ml
Immunohistochemistry-Paraffin: PLEKHM2 Antibody [NBP1-77089] - Human liver tissue with PLEKHM2 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PLEKHM2 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PLEKHM2. The immunogen is located within the last 50 amino acids of PLEKHM2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLEKHM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
PLEKHM2 Peptide (NBP1-77089PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PLEKHM2 Antibody

  • KIAA0842RP11-169K16.1
  • novel RUN and PH domain-containing protein
  • PH domain-containing family M member 2
  • pleckstrin homology domain containing, family M (with RUN domain) member 2
  • pleckstrin homology domain-containing family M member 2
  • Salmonella-induced filaments A and kinesin-interacting protein
  • SifA (Salmonella-induced filaments A) and kinesin-interacting protein
  • SifA and kinesin-interacting protein
  • SKIP

Background

PLEKHM2, also known as SKIP, is a member of the M family of Pleckstrin homology domain-containing proteins. While little is known of PLEKHM2, a recent study examining differential gene expression in human hematopoietic stem cells has shown it to be specifically expressed in stem cells, suggesting that PLEKHM2 may play a role in erythroid commitment and development. Other studies have shown that PLEKHM2 is required for interaction with the Salmonella virulence factor SifA for Salmonella pathogenesis, suggesting that PLEKHM2 has cellular roles other than in the developing embryo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLEKHM2