SKIP Antibody - BSA Free

Western Blot: SKIP Antibody [NBP2-94173] - Analysis of extracts of Mouse skeletal muscle, using INPP5K antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.

Immunocytochemistry/Immunofluorescence: SKIP Antibody [NBP2-94173] - Analysis of PC-12 cells using INPP5K Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Western Blot: SKIP Antibody [NBP2-94173] - Analysis of extracts of 293T cells, using INPP5K antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry/ Immunofluorescence: SKIP Antibody - BSA Free [NBP2-94173] - Immunofluorescence analysis of HeLa cells using SKIP Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: SKIP Antibody - BSA Free [NBP2-94173] - Immunofluorescence analysis of A-549 cells using SKIP Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: SKIP Antibody - BSA Free [NBP2-94173] - Immunofluorescence analysis of NIH/3T3 cells using SKIP Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SKIP Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SKIP Antibody - BSA Free (NBP2-94173) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 149-448 of human INPP5K (NP_057616.2). HLPPHISNNYQRLEHFDRILEMQNCEGRDIPNILDHDLIIWFGDMNFRIEDFGLHFVRESIKNRCYGGLWEKDQLSIAKKHDPLLREFQEGRLLFPPTYKFDRNSNDYDTSEKKRKPAWTDRILWRLKRQPCAGPDTPIPPASHFSLSLRGYSSHMTYGISDHKPVSGTFDLELKPLVSAPLIVLMPEDLWTVENDMMVSYSSTSDFPSSPWDWIGLYKVGLRDVNDYVSYAWVGDSKVSCSDNLNQVYIDISNIPTTEDEFLLCYYSNSLRSVVGISRPFQIPPGSLREDPLGEAQPQI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INPP5K
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SKIP Antibody - BSA Free

• EC 3.1.3
• EC 3.1.3.56
• inositol polyphosphate-5-phosphatase K
• PPS
• skeletal muscle and kidney enriched inositol phosphatase
• Skeletal muscle and kidney-enriched inositol phosphatase
• SKIPinositol polyphosphate 5-phosphatase K

Background

Inositol polyphosphate-5-phosphatase K encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in regions lacking actin stress fibers. It is thought that this protein may negatively regulate the actin cytoskeleton. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INPP5K