![Western Blot: SLC6A12 Antibody [NBP1-88641] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/SLC6A12-Antibody-Western-Blot-NBP1-88641-img0007.jpg "Western Blot: SLC6A12 Antibody [NBP1-88641] - Analysis in human cell line HepG2.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit SLC6A12 Antibody - BSA Free (NBP1-88641) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-SLC6A12 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: TRGPFRKRLRQLITPDSSLPQPKQHPCLDGSAGRNFGPSPTREGLIAGEKETHL |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC6A12 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SLC6A12 Antibody (NBP1-88641)Find related products by research area.
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| Gene Symbol | SLC6A12 |
![Immunocytochemistry/Immunofluorescence: SLC6A12 Antibody [NBP1-88641] - Immunofluorescent cytostaining. Staining with rabbit anti-SLC6A12 (AlexaFluor 488, green) and goat anti-AKR1B1 (AlexaFluor594, red) in cultured healthy human myotubes at different time points. Myotubes treated with 300 u/ml IFNgamma and 30 ng/ml TNFalpha display low levels of SLC6A12 that is increased at the 48 h time point. Levels return back to constitutive low levels after 72 h, with staining levels similar to those in untreated cells. Staining for AKR1B1 shows continuously high levels between 24 and 72 h. In untreated cells, AKR1B1 expression levels are substantially lower. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fneur.2018.00846/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SLC6A12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88641-img0008.jpg "Immunocytochemistry/Immunofluorescence: SLC6A12 Antibody [NBP1-88641] - Immunofluorescent cytostaining. Staining with rabbit anti-SLC6A12 (AlexaFluor 488, green) and goat anti-AKR1B1 (AlexaFluor594, red) in cultured healthy human myotubes at different time points. Myotubes treated with 300 u/ml IFNgamma and 30 ng/ml TNFalpha display low levels of SLC6A12 that is increased at the 48 h time point. Levels return back to constitutive low levels after 72 h, with staining levels similar to those in untreated cells. Staining for AKR1B1 shows continuously high levels between 24 and 72 h. In untreated cells, AKR1B1 expression levels are substantially lower. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fneur.2018.00846/full), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: SLC6A12 Antibody [NBP1-88641] - Staining of human kidney shows moderate cytoplasmic and luminal membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/SLC6A12-Antibody-Immunohistochemistry-Paraffin-NBP1-88641-img0005.jpg "Immunohistochemistry-Paraffin: SLC6A12 Antibody [NBP1-88641] - Staining of human kidney shows moderate cytoplasmic and luminal membranous positivity in cells in tubules.")
![Immunocytochemistry/Immunofluorescence: SLC6A12 Antibody [NBP1-88641] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SLC6A12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88641-img0006.jpg "Immunocytochemistry/Immunofluorescence: SLC6A12 Antibody [NBP1-88641] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.")
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")