SLC6A12 Antibody - BSA Free

Western Blot: SLC6A12 Antibody [NBP1-88641] - Analysis in human cell line HepG2.

SLC6A12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88641-img0008.jpg

Immunocytochemistry/Immunofluorescence: SLC6A12 Antibody [NBP1-88641] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.

Immunofluorescent cytostaining. (A) Staining with mouse anti-SLC6A12 (AlexaFluor 594, red) and rabbit anti-AKR1B1 (AlexaFluor488, green) in CCL-136 cells after 24 h treatment. Untreated control cells shows low levels of SLC6A12 and AKR1B1. Treatment with 30 ng/ml TNF alpha markedly increases AKR1B1 levels. Treatment with 300 u/ml IFN gamma and 30 ng/ml TNF alpha strongly increases both SLC6A12 levels and AKR1B1 protein levels. Addition of 100 mM NaCl to the medium also increases both SLC6A12 and AKR1B1 protein expression. (B) Staining with rabbit anti-SLC6A12 (AlexaFluor 488, green) and goat anti-AKR1B1 (AlexaFluor594, red) in cultured healthy human myotubes at different time points. Myotubes treated with 300 u/ml IFN gamma and 30 ng/ml TNF alpha display low levels of SLC6A12 that is increased at the 48 h time point. Levels return back to constitutive low levels after 72 h, with staining levels similar to those in untreated cells. Staining for AKR1B1 shows continuously high levels between 24 and 72 h. In untreated cells, AKR1B1 expression levels are substantially lower. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30364257), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Staining of human kidney shows strong membranous cytoplasmic positivity in cells in tubules.

Staining of human liver shows strong membranous-cytoplasmic positivity in hepatocytes.

Staining of human prostate shows no positivity in glandular cells as expected.

Staining of human cerebral cortex shows strong membranous cytoplasmic positivity in endothelial cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SLC6A12 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TRGPFRKRLRQLITPDSSLPQPKQHPCLDGSAGRNFGPSPTREGLIAGEKETHL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC6A12
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
• Control Peptide:

  SLC6A12 Protein (NBP1-88641PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SLC6A12 Antibody - BSA Free

• BGT1
• BGT-1
• FLJ38727
• gamma-aminobutyric acid transporter
• SLC6A12
• solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12

Background

Transports betaine and GABA. May have a role in regulation of GABAergic transmission in the brain through the reuptake of GABA into presynaptic terminals, as well as in osmotic regulation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC6A12 Antibody (NBP1-88641)(3)

Publications using NBP1-88641

• Xie Y, Yang F, He L, Huang H et Al. Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier Neuron 2024-08-27 [PMID: 39191260]
• C Merckx, G Cosemans, J Zschüntzsc, R Raedt, J Schmidt, B De Paepe, JL De Bleecke Description of Osmolyte Pathways in Maturing Mdx Mice Reveals Altered Levels of Taurine and Sodium/Myo-Inositol Co-Transporters International Journal of Molecular Sciences, 2022-03-17;23(6):. 2022-03-17 [PMID: 35328671] (Immunocytochemistry/ Immunofluorescence, Human)
• De Paepe B, Zschuntzsch J, Šokčević T et al. Induction of Osmolyte Pathways in Skeletal Muscle Inflammation: Novel Biomarkers for Myositis. Front Neurol. 2018-10-11 [PMID: 30364257] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: SLC6A12