| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88641-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05085-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89282-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03772-20ul | |||
![Western Blot: SLC6A12 Antibody [NBP1-88641] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/SLC6A12-Antibody-Western-Blot-NBP1-88641-img0007.jpg "Western Blot: SLC6A12 Antibody [NBP1-88641] - Analysis in human cell line HepG2.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SLC6A12 Antibody [NBP1-88641] - Immunofluorescent cytostaining. Staining with rabbit anti-SLC6A12 (AlexaFluor 488, green) and goat anti-AKR1B1 (AlexaFluor594, red) in cultured healthy human myotubes at different time points. Myotubes treated with 300 u/ml IFNgamma and 30 ng/ml TNFalpha display low levels of SLC6A12 that is increased at the 48 h time point. Levels return back to constitutive low levels after 72 h, with staining levels similar to those in untreated cells. Staining for AKR1B1 shows continuously high levels between 24 and 72 h. In untreated cells, AKR1B1 expression levels are substantially lower. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fneur.2018.00846/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SLC6A12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88641-img0008.jpg)
![Immunohistochemistry-Paraffin: SLC6A12 Antibody [NBP1-88641] - Staining of human kidney shows moderate cytoplasmic and luminal membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/SLC6A12-Antibody-Immunohistochemistry-Paraffin-NBP1-88641-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SLC6A12 Antibody [NBP1-88641] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SLC6A12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88641-img0006.jpg)
![Western Blot: SLC6A12 Antibody [NBP2-26172] - Staining of Human Olfactory bulb lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SLC6A12-Antibody-Western-Blot-NBP2-26172-img0001.jpg "Western Blot: SLC6A12 Antibody [NBP2-26172] - Staining of Human Olfactory bulb lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: SLC6A12 Overexpression Lysate (Adult Normal) [NBP2-09410] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC6A12.](http://images.novusbio.com/fullsize/SLC6A12-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09410-img0001.jpg "Western Blot: SLC6A12 Overexpression Lysate (Adult Normal) [NBP2-09410] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC6A12.")
![Western Blot: SLC6A12 Antibody [NBP3-05085] - Analysis of extracts of 293T cells, using SLC6A12 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .](http://images.novusbio.com/fullsize/SLC6A12-Antibody-Western-Blot-NBP3-05085-img0001.jpg "Western Blot: SLC6A12 Antibody [NBP3-05085] - Analysis of extracts of 293T cells, using SLC6A12 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .")
![Immunocytochemistry/Immunofluorescence: KCC3/SLC12A6 Antibody [NBP1-89282] - Staining of human cell line A-431 shows localization to cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/KCC3-SLC12A6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89282-img0005.jpg "Immunocytochemistry/Immunofluorescence: KCC3/SLC12A6 Antibody [NBP1-89282] - Staining of human cell line A-431 shows localization to cytosol & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: KCC3/SLC12A6 Antibody [NBP1-89282] - Staining of human Lymph node shows moderate membranous and cytoplasmic positivity in germinal and non-germinal center cells.](http://images.novusbio.com/fullsize/KCC3-SLC12A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89282-img0011.jpg)
![Immunohistochemistry-Paraffin: KCC3/SLC12A6 Antibody [NBP1-89282] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/KCC3-SLC12A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89282-img0007.jpg)
![Immunohistochemistry-Paraffin: KCC3/SLC12A6 Antibody [NBP1-89282] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/KCC3-SLC12A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89282-img0009.jpg)
![Immunohistochemistry-Paraffin: KCC3/SLC12A6 Antibody [NBP1-89282] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/KCC3-SLC12A6-Antibody-Immunohistochemistry-Paraffin-NBP1-89282-img0010.jpg)
![Western Blot: KCC3/SLC12A6 Antibody [NBP1-06044] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/KCC3-SLC12A6-Antibody-Western-Blot-NBP1-06044-img0005.jpg "Western Blot: KCC3/SLC12A6 Antibody [NBP1-06044] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: KCC3/SLC12A6 Antibody [NBP1-06044] - (2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/KCC3-SLC12A6-Antibody-Immunohistochemistry-Paraffin-NBP1-06044-img0004.jpg)
![SDS-Page: Recombinant Human KCC3/SLC12A6 Protein [H00009990-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00009990-Q01-1.jpg "SDS-Page: Recombinant Human KCC3/SLC12A6 Protein [H00009990-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot:[NBP3-30600]-Detection of human KCC3 by western blot. Samples: Whole cell lysate prepared using RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-KCC3 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30600_rabbit-kcc3-slc12a6-pab-23120251225644.jpg "Western Blot:[NBP3-30600]-Detection of human KCC3 by western blot. Samples: Whole cell lysate prepared using RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-KCC3 antibody used for WB at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: KCC3/SLC12A6 Antibody [NBP3-03772] - Analysis of U2OS cells using KCC3/SLC12A6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KCC3-SLC12A6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03772-img0001.jpg "Immunocytochemistry/Immunofluorescence: KCC3/SLC12A6 Antibody [NBP3-03772] - Analysis of U2OS cells using KCC3/SLC12A6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Western Blot: SLC12A6/KCC3 Overexpression Lysate (Adult Normal) [NBP2-10905] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC12A6/KCC3.](http://images.novusbio.com/fullsize/SLC12A6-KCC3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10905-img0001.jpg "Western Blot: SLC12A6/KCC3 Overexpression Lysate (Adult Normal) [NBP2-10905] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC12A6/KCC3.")
![Western Blot: SLC12A6/KCC3 Overexpression Lysate (Adult Normal) [NBP2-10904] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC12A6/KCC3.](http://images.novusbio.com/fullsize/SLC12A6-KCC3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10904-img0001.jpg "Western Blot: SLC12A6/KCC3 Overexpression Lysate (Adult Normal) [NBP2-10904] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SLC12A6/KCC3.")
![Immunoprecipitation:KCC3/SLC12A6 AntibodyNBP3-29424] -Detection of human KCC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-KCC3 antibody NBP3-29424 (lot NBP3-29424-1) used for IP at 6 µg per reaction. KCC3 was also immunoprecipitated by rabbit anti-KCC3 antibody for blotting immunoprecipitated KCC3, A304-409A-1 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29424_rabbit-kcc3-slc12a6-pab-3012202415241410.jpg "Immunoprecipitation:KCC3/SLC12A6 AntibodyNBP3-29424] -Detection of human KCC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-KCC3 antibody NBP3-29424 (lot NBP3-29424-1) used for IP at 6 µg per reaction. KCC3 was also immunoprecipitated by rabbit anti-KCC3 antibody for blotting immunoprecipitated KCC3, A304-409A-1 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")