CTR2 Antibody

Images

 
Western Blot: CTR2 Antibody [NBP1-05199] - Detection of CTR2 in MEF cell lysate.
Simple Western: CTR2 Antibody [NBP1-05199] - Simple Western lane view shows a specific band for CTR2 in 0.5 mg/ml of MEF (left) and Human Placenta (right) lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.93 mg/ml

CTR2 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human CTR2 (within residues 50-100). [Swiss-Prot# O15432]
Localization
Membrane; Multi-pass membrane protein (probable)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC31A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Simple Western 10 ug/ml
Application Notes
This CTR2 antibody is useful for Western blot, where a band is seen at approx. 71 kDa. The theoretical molecular weight of CTR2 is 15 kDa. The difference between the expected size and the observed size in Western blot is believed to be due to a multimeric form of CTR2 (Bertinato et al).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
MEF Whole Cell Lysate
Control Peptide
CTR2 Peptide (NBP1-05199PEP)
Publications
Read Publications using
NBP1-05199 in the following applications:

  • WB
    3 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
0.93 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CTR2 Antibody

  • Copper transporter 2
  • COPT2SLC13A2
  • CTR2Solute carrier family 31 member 2
  • hCTR2probable low affinity copper uptake protein 2
  • solute carrier family 31 (copper transporters), member 2

Background

CTR2 (copper transporter 2) belongs to copper transporter (Ctr) (TC 1.A.56) family/SLC31A subfamily and it is a second Cu permease enzyme with high similarity in sequence as well as in overall organization to CTR1 i.e. 3 putative transmembrane domains with an intramembranous MX3M motif, however, it does not have an extended N-terminal domain. CTR2 localizes to intracellular compartments, most notably the endosome or lysosome and it mediates Cu transport into the cytoplasm based on measures of elevated Cu accumulation or metal responsive reporter activation in cells in which CTR2 levels have been altered.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CTR2 Antibody (NBP1-05199)(3)

Reviews for CTR2 Antibody (NBP1-05199) (0)

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Product General Protocols

View specific protocols for CTR2 Antibody (NBP1-05199): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CTR2 Antibody (NBP1-05199). (Showing 1 - 1 of 1 FAQs).

  1. I have searched your website to find ctr2 antibody for my rat experiment(mainly about IHC &WB), however, I found most ctr2 antibody used for mouse and human not for rat.Could you help me to check the information on ctr2 antibody ?Thanks a lot!
    • We have two antibodies to CTR2 - NBP1-05199 which is validated for Western blotting using samples from human and mouse, and NBP1-85512 which is validated for IHC-P using human samples. Human and mouse CTR2 share a 77% sequence homology, however I am unable to align either of these sequence with that of the rat protein, since the rat sequence is not available on UniProt. If you have the rat sequence I would be happy to perform the alignment for you, email technical@novusbio.com. If you wish to try either of these antibodies in an untested species or application I can strongly recommend our Innovators Reward Program. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. This allows us to gain more information on our products, and enables you to save money.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-05199AF350
Alexa Fluor 405 NBP1-05199AF405
Alexa Fluor 488 NBP1-05199AF488
Alexa Fluor 532 NBP1-05199AF532
Alexa Fluor 594 NBP1-05199AF594
Alexa Fluor 647 NBP1-05199AF647
Alexa Fluor 700 NBP1-05199AF700
Alexa Fluor 750 NBP1-05199AF750
Biotin NBP1-05199B
DyLight 350 NBP1-05199UV
DyLight 405 NBP1-05199V
DyLight 488 NBP1-05199G
DyLight 550 NBP1-05199R
DyLight 594 NBP1-05199DL594
DyLight 650 NBP1-05199C
DyLight 680 NBP1-05199FR
DyLight 755 NBP1-05199IR
HRP NBP1-05199H
Janelia Fluor 549 NBP1-05199JF549
Janelia Fluor 646 NBP1-05199JF646

Additional CTR2 Products

Array NBP1-05199

Bioinformatics Tool for CTR2 Antibody (NBP1-05199)

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Blogs on CTR2

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Contact Information

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Product PDFs

Calculators

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Bioinformatics

Gene Symbol SLC31A2
Entrez
Uniprot