SLC22A1 Antibody - BSA Free

Western Blot: SLC22A1 Antibody [NBP1-59464] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 8.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%

Immunohistochemistry-Paraffin: SLC22A1 Antibody [NBP1-59464] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X magnification.

Western Blot: SLC22A1 Antibody [NBP1-59464] - Jurkat cell lysate, Antibody Titration: 2.5ug/ml

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SLC22A1 Antibody - BSA Free Summary

• Description: The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
• Immunogen: Synthetic peptides corresponding to SLC22A1(solute carrier family 22 (organic cation transporter), member 1) The peptide sequence was selected from the N terminal of SLC22A1 (NP_003048). Peptide sequence LSCVDPLASLATNRSHLPLGPCQDGWVYDTPGSSIVTEFNLVCADSWKLD The peptide sequence for this immunogen was taken from within the described region.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC22A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 4-8 ug/ml
  • Western Blot 1.0 ug/ml
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 2% Sucrose
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SLC22A1 Antibody - BSA Free

• hOCT1
• OCT1
• oct1_cds
• OCT1solute carrier family 22 member 1
• Organic cation transporter 1
• SLC22A1
• solute carrier family 22 (organic cation transporter), member 1

Background

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. SLC22A1 contains twelve putative transmembrane domains and is a plasma integral membrane protein.Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. Two transcript variants encoding two different isoforms have been found for this gene, but only the longer variant encodes a functional transporter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC22A1 Antibody (NBP1-59464)(1)

Publication using NBP1-59464

• Andreev E, Brosseau N, Carmona E et al. The human organic cation transporter OCT1 mediates high affinity uptake of the anticancer drug daunorubicin Sci Rep 2016-02-10 [PMID: 26861753] (WB, Human)

Bioinformatics

• Gene Symbol: SLC22A1
• Entrez:
  • 519461(Bovine)
  • 6580(Human)
  • 20517(Mouse)
  • 100008659(Rabbit)
  • 24904(Rat)
• Uniprot:
  • Q9NQD4(Human)