OATP2/8 Antibody (MDQ)

Images

 
Immunocytochemistry/ Immunofluorescence: OATP2/8 Antibody (MDQ) [NB100-74482] - Analysis of OATP8 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
MDQ
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

OATP2/8 Antibody (MDQ) Summary

Immunogen
Synthetic peptide corresponding to residues M(1) D Q N Q H L N K T A E A Q P S E N K K T R Y(23) C of human OATP2.
Epitope
The exact epitope within the immunizing peptide sequence is unknown.
Specificity
NB100-74482 detects OATP2 and OATP8 from human tissue and cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SLCO1B1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:20
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:20
  • Immunohistochemistry-Paraffin 1:20
  • Immunoprecipitation 1:10 - 1:500
Application Notes
WB usage w as reported in literature (See Cui Y et al.). IHC-P usage reported in scientific literature (PMID: 25063395).
Positive Control
OATP1B3/SLCO1B3/OATP8 Lysate (NBL1-16204)
Publications
Read Publications using
NB100-74482 in the following applications:

  • IHC
    6 publications
  • 2 publications
  • IP
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tissue culture supernatant
Preservative
0.09% Sodium Azide
Purity
Unpurified

Notes

This product was originally labeled as an antibody to OATP8. However, the amino acid sequence used as the immunizing peptide is 100% identical to OATP2. Furthermore, publications using this antibody show reactivity to both OATP2 and 8. As such, this antibody is reactive to both OATP2 and OATP8.

Alternate Names for OATP2/8 Antibody (MDQ)

  • HBLRR
  • LST1
  • LST-1
  • LST-2
  • LST3
  • LST-3TM13
  • OATP1B1
  • OATP1B3
  • OATP2
  • OATP8
  • OATP-8
  • OATPC
  • OATP-C
  • SLC21A6
  • SLC21A8
  • SLCO1B1 solute carrier organic anion transporter family, member 1B1

Background

The OATPs (organic anion transporter polypeptides) are a family of transport proteins that have been found to mediate the uptake of organic ions into hepatocyte cells. These proteins fall into the solute carrier family 21A (SLC21A), and are expressed in liver, kidney, and brain. These polypeptides are involved in the excretion of potentially toxic compounds and therefore play a role in the overall detoxification system of the body. Unlike other members of the OATP family, OATP2 and 8 have been detected exclusively in the liver, and there is an 80% amino acid sequence homology between these polypeptides. Other names for OATP2 include SLC21A6, OATP-C, and LST-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OATP2/8 Antibody (NB100-74482)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: IHC, IHC-P, IP, WB.


Filter By Application
IHC
(6)
IHC-P
(2)
IP
(1)
WB
(2)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-74482 Applications Species
Imada S, Kobayashi T, Kitao A et al. Central bisectionectomy for hepatocellular carcinoma in a patient with indocyanine green excretory defect associated with reduced expression of the liver transporter. Surg Case Rep Dec 1 2016 [PMID: 27589984] (IHC) IHC
Thomson MM, Hines RN, Schuetz EG, Meibohm B. Expression Patterns of OATP 1B1 and OATP 1B3 Protein in Human Pediatric Liver. Drug Metab. Dispos. Apr 20 2016 [PMID: 27098745] (WB, Human) WB Human
Fujita N, Nishie A, Asayama Y et al. Significance of the Signal Intensity of Gadoxetic Acid-enhanced MR Imaging for Predicting the Efficacy of Hepatic Arterial Infusion Chemotherapy in Hepatocellular Carcinoma. Magn Reson Med Sci 2015 Sep 04 [PMID: 26346403] (IHC-P, Human) IHC-P Human
Kitao A, Matsui O, Yoneda N et al. Hepatocellular Carcinoma with beta-Catenin Mutation: Imaging and Pathologic Characteristics Radiology. 2015 Feb 06 [PMID: 25668519] (Human) Human
Kagawa T, Oka A, Kobayashi Y et al. Recessive inheritance of population-specific intronic LINE-1 insertion causes a Rotor syndrome phenotype. Hum. Mutat. 2014 Dec 25 [PMID: 25546334] (IHC, Human) IHC Human
Fujita N, Nishie A, Kubo Y et al. Hepatocellular carcinoma: clinical significance of signal heterogeneity in the hepatobiliary phase of gadoxetic acid-enhanced MR imaging. Eur Radiol 2014 Jul 26 [PMID: 25063395] (IHC-P, Human)

Details:
OATP8 or OATP2/8 antibody (clone MDQ) used for IHC-P on 10 % formalin fixed tissues of human hepatocellular carcinoma/HCC - 1:100 dilution, streptavidin-biotin-peroxidase - DAB detection method. IHC images documented on the tumour cells with no OATP8 expression (grade 0, Figure 3d), decreased expression of OATP8 (grade 1, Figure 4d) and increased expression of OATP8 (grade 3, Figure 5d).
IHC-P Human
Ohama H, Imai Y, Nakashima O et al. Images of Sonazoid-enhanced ultrasonography in multistep hepatocarcinogenesis: comparison with Gd-EOB-DTPA-enhanced MRI. J Gastroenterol 2013 Aug 1 [PMID: 23903870] (IHC, Human) IHC Human
Yoneda N, Matsui O, Kitao A, Kita R et al. Hypervascular hepatocellular carcinomas showing hyperintensity on hepatobiliary phase of gadoxetic acid-enhanced magnetic resonance imaging: a possible subtype with mature hepatocyte nature. Jpn J Radiol 2013 Jul [PMID: 23771695] (IHC, Human) IHC Human
Kitao A, Matsui O, Yoneda N et al. Hypervascular Hepatocellular Carcinoma: Correlation between Biologic Features and Signal Intensity on Gadoxetic Acid-enhanced MR Images Radiology 2012 Dec [PMID: 23175543]
Kitao A, Matsui O, Yoneda N, Kozaka K, Shinmura R, Koda W, Kobayashi S, Gabata T, Zen Y, Yamashita T, Kaneko S, Nakanuma Y. The uptake transporter OATP8 expression decreases during multistep hepatocarcinogenesis: correlation with gadoxetic acid enhanced MR imaging. Eur Radiol;21(10):2056-66. 2011 Oct. [PMID: 21626360] (IHC, Human) IHC Human
Show All 12 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OATP2/8 Antibody (NB100-74482). (Showing 1 - 1 of 1 FAQs).

  1. The customer is very confused with product name of #NB100-74482. SLC01B3(MDQ/5F260) was indicated on the vial label. SLC01B3 is alternative name of OATP8. But OATP2(MDQ) was indicated on the datasheet on your web. Furthermore, when the customer searched 5F260 on your web, #NB600-1158 (OATP8 Antibody (5F260)) appeared. I think something is wrong for #NB100-74482. Could you confirm the product name of suppliers' and let us know? As far as I looked the Internet, Clone MDQ reacts with both OATP2 and OATP8. Clone 5F260 also reacts with both OATP2 and OATP8? Is MDQ identical with 5F260?
    • Clone MDQ and clone 5F260 are two separate clones generated using the same immunogen. Both are also predicted to react with both OATP2 and OATP8, based on the sequence similarity.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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OATP2/8 NB100-74482

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Bioinformatics

Gene Symbol SLCO1B1
Entrez
Uniprot