OATP2/8 Antibody (MDQ)

Immunocytochemistry/Immunofluorescence: OATP2/8 Antibody (MDQ) [NB100-74482] - Analysis of OATP8 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a OATP8 monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. OATP8 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry: OATP2/8 Antibody (MDQ) [NB100-74482] - Immunohistochemical staining of liver transporters in the resected specimens from our case and control cases. Image collected and cropped by CiteAb from the following publication (surgicalcasereports.springeropen.com/articles/10.1186/s40792-016-0216-8), licensed under a CC-BY license.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clone: MDQ
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

OATP2/8 Antibody (MDQ) Summary

• Description: This product was originally labeled as an antibody to OATP8. However, the amino acid sequence used as the immunizing peptide is 100% identical to OATP2. Furthermore, publications using this antibody show reactivity to both OATP2 and 8. As such, this antibody is reactive to both OATP2 and OATP8.
• Immunogen: Synthetic peptide corresponding to residues M(1) D Q N Q H L N K T A E A Q P S E N K K T R Y(23) C of human OATP2.
• Epitope: The exact epitope within the immunizing peptide sequence is unknown.
• Specificity: NB100-74482 detects OATP2 and OATP8 from human tissue and cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLCO1B1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:20
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:20
  • Immunohistochemistry-Paraffin 1:20
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: WB usage w as reported in literature (See Cui Y et al.). IHC-P usage reported in scientific literature (PMID: 25063395).
• Control:

  OATP1B3/SLCO1B3/OATP8 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for OATP2/8 Antibody (MDQ)

• HBLRR
• LST1
• LST-1
• LST-2
• LST3
• LST-3TM13
• OATP1B1
• OATP1B3
• OATP2
• OATP8
• OATP-8
• OATPC
• OATP-C
• SLC21A6
• SLC21A8
• SLCO1B1 solute carrier organic anion transporter family, member 1B1

Background

The OATPs (organic anion transporter polypeptides) are a family of transport proteins that have been found to mediate the uptake of organic ions into hepatocyte cells. These proteins fall into the solute carrier family 21A (SLC21A), and are expressed in liver, kidney, and brain. These polypeptides are involved in the excretion of potentially toxic compounds and therefore play a role in the overall detoxification system of the body. Unlike other members of the OATP family, OATP2 and 8 have been detected exclusively in the liver, and there is an 80% amino acid sequence homology between these polypeptides. Other names for OATP2 include SLC21A6, OATP-C, and LST-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OATP2/8 Antibody (NB100-74482)(14)

Publications using NB100-74482

• Kiander W, Vellonen KS, Malinen MM Et al. The Effect of Single Nucleotide Variations in the Transmembrane Domain of OATP1B1 on in vitro Functionality Pharmaceutical research 2021-10-13 [PMID: 34647232] (ICC/IF, Human)
• Yoneda N, Matsui O, Kitao A et al. Peri-tumoral hyperintensity on hepatobiliary phase of gadoxetic acid-enhanced MRI in hepatocellular carcinomas: correlation with peri-tumoral hyperplasia and its pathological features. Abdom Radiol (NY) 2017-12-19 [PMID: 29260280] (IF/IHC, Human)
• Imada S, Kobayashi T, Kitao A et al. Central bisectionectomy for hepatocellular carcinoma in a patient with indocyanine green excretory defect associated with reduced expression of the liver transporter. Surg Case Rep 2016-12-01 [PMID: 27589984] (IF/IHC)
• Thomson MM, Hines RN, Schuetz EG, Meibohm B. Expression Patterns of OATP 1B1 and OATP 1B3 Protein in Human Pediatric Liver. Drug Metab. Dispos. 2016-04-20 [PMID: 27098745] (WB, Human)
• Fujita N, Nishie A, Asayama Y et al. Significance of the Signal Intensity of Gadoxetic Acid-enhanced MR Imaging for Predicting the Efficacy of Hepatic Arterial Infusion Chemotherapy in Hepatocellular Carcinoma. Magn Reson Med Sci 2015-09-04 [PMID: 26346403] (IHC-P, Human)
• Kitao A, Matsui O, Yoneda N et al. Hepatocellular Carcinoma with beta-Catenin Mutation: Imaging and Pathologic Characteristics Radiology. 2015-02-06 [PMID: 25668519] (Human)
• Kagawa T, Oka A, Kobayashi Y et al. Recessive inheritance of population-specific intronic LINE-1 insertion causes a Rotor syndrome phenotype. Hum. Mutat. 2014-12-25 [PMID: 25546334] (IF/IHC, Human)
• Fujita N, Nishie A, Kubo Y et al. Hepatocellular carcinoma: clinical significance of signal heterogeneity in the hepatobiliary phase of gadoxetic acid-enhanced MR imaging. Eur Radiol 2014-07-26 [PMID: 25063395] (IHC-P, Human)
  
  Details:
  OATP8 or OATP2/8 antibody (clone MDQ) used for IHC-P on 10 % formalin fixed tissues of human hepatocellular carcinoma/HCC - 1:100 dilution, streptavidin-biotin-peroxidase - DAB detection method. IHC images documented on the tumour cells with no OATP8 expression (grade 0, Figure 3d), decreased expression of OATP8 (grade 1, Figure 4d) and increased expression of OATP8 (grade 3, Figure 5d).
• Ohama H, Imai Y, Nakashima O et al. Images of Sonazoid-enhanced ultrasonography in multistep hepatocarcinogenesis: comparison with Gd-EOB-DTPA-enhanced MRI. J Gastroenterol 2013-08-01 [PMID: 23903870] (IF/IHC, Human)
• Yoneda N, Matsui O, Kitao A, Kita R et al. Hypervascular hepatocellular carcinomas showing hyperintensity on hepatobiliary phase of gadoxetic acid-enhanced magnetic resonance imaging: a possible subtype with mature hepatocyte nature. Jpn J Radiol 2013-07-01 [PMID: 23771695] (IF/IHC, Human)
• Kitao A, Matsui O, Yoneda N et al. Hypervascular Hepatocellular Carcinoma: Correlation between Biologic Features and Signal Intensity on Gadoxetic Acid-enhanced MR Images Radiology 2012-12-01 [PMID: 23175543]
• Kitao A, Matsui O, Yoneda N, Kozaka K, Shinmura R, Koda W, Kobayashi S, Gabata T, Zen Y, Yamashita T, Kaneko S, Nakanuma Y. The uptake transporter OATP8 expression decreases during multistep hepatocarcinogenesis: correlation with gadoxetic acid enhanced MR imaging. Eur Radiol;21(10):2056-66. 2011-10-01 [PMID: 21626360] (IF/IHC, Human)
• Kitao A, Zen Y, Matsui O et al. Hepatocellular carcinoma: signal intensity at gadoxetic acid-enhanced MR Imaging--correlation with molecular transporters and histopathologic features. Radiology 2010-09-01 [PMID: 20663969] (Human)
• Cui Y, Konig J, Nies AT et al. Detection of the human organic anion transporters SLC21A6 (OATP2) and SLC21A8 (OATP8) in liver and hepatocellular carcinoma. Lab Invest. 2003-04-01 [PMID: 12695556] (IP, IF/IHC, WB)

FAQs for OATP2/8 Antibody (NB100-74482). (Showing 1 - 1 of 1 FAQs).

1. The customer is very confused with product name of #NB100-74482. SLC01B3(MDQ/5F260) was indicated on the vial label. SLC01B3 is alternative name of OATP8. But OATP2(MDQ) was indicated on the datasheet on your web. Furthermore, when the customer searched 5F260 on your web, #NB600-1158 (OATP8 Antibody (5F260)) appeared. I think something is wrong for #NB100-74482. Could you confirm the product name of suppliers' and let us know? As far as I looked the Internet, Clone MDQ reacts with both OATP2 and OATP8. Clone 5F260 also reacts with both OATP2 and OATP8? Is MDQ identical with 5F260?
   • Clone MDQ and clone 5F260 are two separate clones generated using the same immunogen. Both are also predicted to react with both OATP2 and OATP8, based on the sequence similarity.

Bioinformatics

• Gene Symbol: SLCO1B1
• Entrez:
  • 10599(Human)
  • 28234(Human)
• Uniprot:
  • Q9Y6L6(Human)