MPHOSPH6 Antibody

Images

 
Western Blot: MPHOSPH6 Antibody [NBP2-19388] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MPHOSPH6 Antibody [NBP2-19388] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MPHOSPH6 Antibody [NBP2-19388] - Immunohistochemical analysis of paraffin-embedded MB157 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MPHOSPH6 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MPHOSPH6. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MPHOSPH6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for MPHOSPH6 Antibody

  • M-phase phosphoprotein 6
  • MPP-6
  • MPP6MPP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MPHOSPH6 Antibody (NBP2-19388). (Showing 1 - 1 of 1 FAQ).

  1. I would like to ask about the western blot picture shown on your product page for NBP2-19388, the MPHOSPH6 antibody. The expected molecular weight of MPHOPSH6 should be 19 kDa, however, there are two bands on the western blot, one corresponding to 19 kDa
    • With regard to our product NBP2-19388, we have unfortunately not yet identified the larger band at 55kDa seen in HCT116 cell lysates. The band is much larger than the expected band of 19kDa, and our lab thinks that the band is non-specific.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MPHOSPH6
Uniprot