SLC22A2/OCT2 Antibody (640438) [Unconjugated]

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" > DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" title="SLC22A2/OCT2 was detected in immersion fixed paraffin-embedded sections of human Kidney using Mouse Anti-Human SLC22A2/OCT2, Monoclonal Antibody (Catalog # MAB6547) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" />

SLC22A2/OCT2 was detected in immersion fixed paraffin-embedded sections of human Kidney using Mouse Anti-Human SLC22A2/OCT2, Monoclonal Antibody (Catalog # MAB6547) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="SLC22A2 was detected in immersion fixed paraffin-embedded sections of human kidney using Human SLC22A2 Monoclonal Antibody (Catalog # MAB6547) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

SLC22A2 was detected in immersion fixed paraffin-embedded sections of human kidney using Human SLC22A2 Monoclonal Antibody (Catalog # MAB6547) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol. " > F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol. " title="HEK293 human embryonic kidney cell line transfected with EGP and (A) human SLC22A2/OCT2 or (B) irrelevant protein was stained with Mouse Anti-Human SLC22A2/OCT2 Monoclonal Antibody (Catalog # MAB6547) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol. " />

HEK293 human embryonic kidney cell line transfected with EGP and (A) human SLC22A2/OCT2 or (B) irrelevant protein was stained with Mouse Anti-Human SLC22A2/OCT2 Monoclonal Antibody (Catalog # MAB6547) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

• Reactivity: Hu
• Applications: Flow, IHC, mIF
• Clone: 640438
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SLC22A2/OCT2 Antibody (640438) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human SLC-22A2
  
  Accession # O15244
• Specificity: Detects human SLC22A2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC22A2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Multiplex Immunofluorescence 20 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLC22A2/OCT2 Antibody (640438) [Unconjugated]

• hOCT2
• MGC32628
• OCT2
• OCT2Organic cation transporter 2
• SLC22A2
• solute carrier family 22 (organic cation transporter), member 2
• solute carrier family 22 member 2

Background

Solute carrier family 22 member 2 (SLC22A2; also hOCT2) is a 65 kDa member of the major facilitator superfamily and organic cation transporter family of proteins.   Human SLC22A2 is synthesized as a multipass transmembrane protein that is 555 amino acids (aa) in length.  Human SLC22A2 contains one potential site for N-linked glycosylation.  There are also two additional isoforms for human SLC22A2.   Isoform 2 has a 57 aa substitution for aa 427-483 and a deletion of the 72 aa at positions 484-555.   Isoform 3 has an 18 aa substitution for aa 225-242 and a deletion of residues 243-555.  SLC22A2 has its highest expression in the kidney.  It is also expressed at lower levels in neurons of the cerebral cortex and in various subcortical nuclei.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLC22A2/OCT2 Antibody (MAB6547)(3)

Publications using MAB6547

• Tang, P;Keshi, E;Wilken, S;Wutsdorff, L;Mougnekabol, J;Pratschke, J;Sauer, IM;Haep, N; Generation of an induced pluripotent stem cell (iPSC) line (EXSURGi001-A) from a patient homozygous for the p.Ala165Thr mutation in the MTARC1 gene Stem cell research 2024-10-01 [PMID: 39094505] (Immunohistochemistry, Human)
• Xiaoli Zheng, Yanqing Liu, Qinqin Yu, Hua Wang, Fuqing Tan, Qianying Zhu et al. Response to Comment on “Epigenetic activation of the drug transporter OCT2 sensitizes renal cell carcinoma to oxaliplatin” Science Translational Medicine 2017-05-24 [PMID: 28539476]
• Stefan Winter, Pascale Fisel, Florian Büttner, Anne T. Nies, Arnulf Stenzl, Jens Bedke et al. Comment on “Epigenetic activation of the drug transporter OCT2 sensitizes renal cell carcinoma to oxaliplatin” Science Translational Medicine 2017-05-24 [PMID: 28539468] (Immunohistochemistry, Human)

Bioinformatics

• Gene Symbol: SLC22A2
• Uniprot:
  • O15244()