OCT1 Antibody

Images

 
Western Blot: OCT1 Antibody [NBP2-21584] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OCT1 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: OCT1 Antibody [NBP2-21584] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT1 protein stained by OCT1 antibody diluted at 1:500. Red: phalloidin, a ...read more
Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-21584] - Rat brain. OCT1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-21584] - Hela xenograft, using OCT1 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunoprecipitation: OCT1 Antibody [NBP2-21584] - 293T whole cell lysate/extract A : Control with 2.5 ug of pre-immune rabbit IgG B : Immunoprecipitation of OCT1 by 2.5 ug of OCT1 antibody 7.5% SDS-PAGE The ...read more
Immunoprecipitation: OCT1 Antibody [NBP2-21584] - Immunoprecipitation of OCT1 protein from 293T whole cell extracts using 5 ug of OCT1 antibody. Western blot analysis was performed using OCT1 antibody. EasyBlot ...read more

Product Details

Summary
Reactivity Hu, Rt, Mu, Po, Bv, Ch, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

OCT1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human OCT1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (90%), Porcine (97%), Bovine (95%), Primate (99%), Chicken (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POU2F1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
OCT1 Knockout HeLa Cell Lysate

Reactivity Notes

Chimpanzee (99%), Xenopus laevis (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for OCT1 Antibody

  • FLJ42836
  • NF-A1
  • Oct-1
  • OCT1class 2, transcription factor 1
  • OTF1
  • POU class 2 homeobox 1
  • POU domain class 2, transcription factor 1
  • POU2F1

Background

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OCT1 Antibody (NBP2-21584) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol POU2F1