SIX1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SIX1 Antibody [NBP1-84264] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human salivary gland shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human skeletal muscle shows strong nuclear positivity in striated muscle fibers.

Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SIX1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Analysis in human skeletal muscle and liver tissues using HPA001893 antibody. Corresponding SIX1 RNA-seq data are presented for the same tissues.

Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Staining of human skin shows no nuclear positivity in squamous epithelial cells as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ChIP, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SIX1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SIX1 Antibody - BSA Free (NBP1-84264) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and ChIP. Anti-SIX1 Antibody: Cited in 18 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDS
• Predicted Species: Rat (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation Reported in scientific literature (PMID: 24852826)
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Knockdown Validated
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SIX1 Protein (NBP1-84264PEP)

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:24852826).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SIX1 Antibody - BSA Free

• BOS3
• deafness, autosomal dominant 23
• DFNA23
• homeobox protein SIX1
• sine oculis homeobox (Drosophila) homolog 1
• sine oculis homeobox homolog 1 (Drosophila)
• Sine oculis homeobox homolog 1
• SIX homeobox 1
• TIP39

Background

The protein encoded by the SIX1 gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIX1 Antibody (NBP1-84264)(18)

Publications using NBP1-84264

• C Wilson, TC Mertens, P Shivshanka, W Bi, SD Collum, N Wareing, J Ko, T Weng, RP Naikawadi, PJ Wolters, P Maire, SS Jyothula, RA Thandavara, D Ren, ND Elrod, EJ Wagner, HJ Huang, BF Dickey, HL Ford, H Karmouty-Q Sine oculis homeobox homolog 1 (Six1) plays a critical role in pulmonary fibrosis JCI Insight, 2022-05-23;0(0):. 2022-05-23 [PMID: 35420997]
• Min WP, Wei XF Nervilifordin F alleviates intestinal ischemia/reperfusion-induced acute lung injury via inhibiting inflammasome and mTOR pathway Auris Nasus Larynx 2020-10-08 [PMID: 33077306] (IHC-P, Human)
• Christina G. Towers, Anna L. Guarnieri, Doug S. Micalizzi et al. The Six1 oncoprotein downregulates p53 via concomitant regulation of RPL26 and microRNA-27a-3p. Nature Communications 2015-01-01 [PMID: 26687066] (WB, Human)
• I Adrados, J Larrasa-Alonso, A Galarreta et al. The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes. Oncogene 2015-10-26 [PMID: 26500063] (ICC/IF, Human)
• Nagel Stefan, Meyer Corinna, Kaufmann Maren et al. Aberrant expression of homeobox gene SIX1 in Hodgkin lymphoma. Oncotarget 2015-01-01 [PMID: 26473286] (WB, Human)
• Kahlert C, Lerbs T, Pecqueux M et al. Overexpression of SIX1 is an independent prognostic marker in stage I-III colorectal cancer. Int. J. Cancer. 2015-05-06 [PMID: 25951369] (IHC-P, Human)
• Kong J, Zhou X, Liu S et al. Overexpression of sineoculis homeobox homolog 1 predicts poor prognosis of hepatocellular carcinoma. Int J Clin Exp Pathol 2014-01-01 [PMID: 25031720] (WB, ICC/IF, Human)
• Sakakibara I, Santolini M, Ferry A et al. Six homeoproteins and a Iinc-RNA at the fast MYH locus lock fast myofiber terminal phenotype. PLoS Genet 2014-05-01 [PMID: 24852826] (Chemotaxis, WB, Mouse)
• Koehler KR, Hashino E. 3D mouse embryonic stem cell culture for generating inner ear organoids. Nat Protoc 2014-06-01 [PMID: 24784820] (IF/IHC)
• Jefferson WN, Chevalier DM, Phelps JY et al. Persistently Altered Epigenetic Marks in the Mouse Uterus After Neonatal Estrogen Exposure. Mol Endocrinol 2013-10-01 [PMID: 24002655] (IF/IHC, Human)
• Le Grand F, Grifone R, Mourikis P et al. Six1 regulates stem cell repair potential and self-renewal during skeletal muscle regeneration. J Cell Biol 2012-09-03 [PMID: 22945933]
• Iwanaga R, Wang CA, Micalizzi DS et al. Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways. Breast Cancer Res 2012-07-01 [PMID: 22765220]
• Smith AL, Iwanaga R, Drasin DJ et al. The miR-106b-25 cluster targets Smad7, activates TGF-Beta signaling, and induces EMT and tumor initiating cell characteristics downstream of Six1 in human breast cancer. Oncogene 2012-12-01 [PMID: 22286770]
• Ono H, Imoto I, Kozaki K et al. SIX1 promotes epithelial-mesenchymal transition in colorectal cancer through ZEB1 activation. Oncogene 2012-11-22 [PMID: 22286765]
• Farabaugh SM, Micalizzi DS, Jedlicka P et al. Eya2 is required to mediate the pro-metastatic functions of Six1 via the induction of TGF-Beta signaling, epithelial-mesenchymal transition, and cancer stem cell properties. Oncogene 2012-02-01 [PMID: 21706047]
• Micalizzi DS et al. The Six1 homeoprotein induces human mammary carcinoma cells to undergo epithelial-mesenchymal transition and metastasis in mice through increasing TGF-beta signaling. J Clin Invest;119(9):2678-90. 2009-09-01 [PMID: 19726885]
• McCoy EL et al. Six1 expands the mouse mammary epithelial stem/progenitor cell pool and induces mammary tumors that undergo epithelial-mesenchymal transition. J Clin Invest;119(9):2663-77. 2009-09-01 [PMID: 19726883]
• Wan F et al. Gene expression changes during HPV-mediated carcinogenesis: a comparison between an in vitro cell model and cervical cancer. Int J Cancer;123(1):32-40. 2008-07-01 [PMID: 18398830]

Bioinformatics

• Gene Symbol: SIX1
• Entrez:
  • 6495(Human)
• Uniprot:
  • Q15475(Human)