SIX1 Antibody

Images

 
Genetic Strategies: Western Blot: SIX1 Antibody [NBP1-84264] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: SIX1 Antibody [NBP1-84264] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining in human skeletal muscle and liver tissues. Corresponding SIX1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human breast cancer shows weak to moderate nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human salivary gland shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human skeletal muscle shows strong nuclear positivity in striated muscle fibers.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SIX1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDS
Specificity
Specificity of human SIX1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. ChIP, WB reported in the scientific literature (PMID: 24852826).
Control Peptide
SIX1 Protein (NBP1-84264PEP)
Publications
Read Publications using
NBP1-84264 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SIX1 Antibody

  • BOS3
  • deafness, autosomal dominant 23
  • DFNA23
  • homeobox protein SIX1
  • sine oculis homeobox (Drosophila) homolog 1
  • sine oculis homeobox homolog 1 (Drosophila)
  • Sine oculis homeobox homolog 1
  • SIX homeobox 1
  • TIP39

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SIX1 Antibody (NBP1-84264)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC, IHC-P, WB.


Filter By Application
ChIP
(1)
ICC/IF
(2)
IHC
(2)
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NBP1-84264 Applications Species
Christina G. Towers, Anna L. Guarnieri, Doug S. Micalizzi et al. The Six1 oncoprotein downregulates p53 via concomitant regulation of RPL26 and microRNA-27a-3p. Nature Communications 2015 [PMID: 26687066] (WB, Human) WB Human
I Adrados, J Larrasa-Alonso, A Galarreta et al. The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes. Oncogene 2015 Oct 26 [PMID: 26500063] (ICC/IF, Human) ICC/IF Human
Nagel Stefan, Meyer Corinna, Kaufmann Maren et al. Aberrant expression of homeobox gene SIX1 in Hodgkin lymphoma. Oncotarget 2015 [PMID: 26473286] (WB, Human) WB Human
Kahlert C, Lerbs T, Pecqueux M et al. Overexpression of SIX1 is an independent prognostic marker in stage I-III colorectal cancer. Int. J. Cancer. 2015 May 06 [PMID: 25951369] (IHC-P, Human) IHC-P Human
Kong J, Zhou X, Liu S et al. Overexpression of sineoculis homeobox homolog 1 predicts poor prognosis of hepatocellular carcinoma. Int J Clin Exp Pathol 2014 [PMID: 25031720] (WB, ICC/IF, Human) WB, ICC/IF Human
Sakakibara I, Santolini M, Ferry A et al. Six homeoproteins and a Iinc-RNA at the fast MYH locus lock fast myofiber terminal phenotype. PLoS Genet 2014 May [PMID: 24852826] (ChIP, WB, Mouse) ChIP, WB Mouse
Koehler KR, Hashino E. 3D mouse embryonic stem cell culture for generating inner ear organoids. Nat Protoc 2014 Jun [PMID: 24784820] (IHC) IHC
Jefferson WN, Chevalier DM, Phelps JY et al. Persistently Altered Epigenetic Marks in the Mouse Uterus After Neonatal Estrogen Exposure. Mol Endocrinol 2013 Oct [PMID: 24002655] (IHC, Human) IHC Human
Le Grand F, Grifone R, Mourikis P et al. Six1 regulates stem cell repair potential and self-renewal during skeletal muscle regeneration. J Cell Biol 2012 Sep 3 [PMID: 22945933]
Iwanaga R, Wang CA, Micalizzi DS et al. Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways. Breast Cancer Res 2012 Jul [PMID: 22765220]
Show All 16 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for SIX1 Antibody (NBP1-84264) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SIX1
Entrez
Uniprot