PTHLH/PTHrP Antibody (677939)

PTHLH/PTHrP was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human PTHLH/PTHrP Monoclonal Antibody (Catalog # MAB6734) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 677939
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PTHLH/PTHrP Antibody (677939) Summary

• Immunogen: E. coli-derived recombinant human PTHLH/PTHrP
  Ala37-Arg175
  Accession # P12272
• Specificity: Detects human PTHLH/PTHrP in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTHLH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PTHLH/PTHrP Antibody (677939)

• BDE2
• HHM
• MGC14611
• Osteostatin
• parathyroid hormone-like hormone
• Parathyroid hormone-like protein
• parathyroid hormone-related protein
• PLPosteostatin
• PTHLH
• PTHR
• PTH-related protein
• PTHRP
• PTH-rP
• PTHRPparathyroid hormone-like related protein

Background

The parathyroid hormone-like hormone (PTHLH), also known as PTHrP, has been recognized in patients suffering from tumors associated with the common neoplastic syndrome humoral hypercalcemia of malignancy (HHM). The 18 kDa protein is a 177 amino acid (aa) precursor that belongs to the parathyroid hormone family. It contains a 24 aa signal sequence followed by a 10 aa propeptide (aa 25-34). The mature chain runs from aa 37-177. Of note, residues 108-129 constitute a nuclear localization signal (NLS) providing evidence that the protein not only acts as a secreted factor, but in an intracrine manner as well. A second isoform is missing aa 176-177, and a 34 aa substitution for aa 176-177 produces a third isoform. There is a strong homology displayed between PTHLH and human parathyroid hormone (PTH) among the first 13 aa. Studies show that the similarity in this area is 67% at the aa and nucleotide levels. Indeed, eight of the first 13 aa of PTHLH and PTH are identical. Human PTHLH and mouse PTHLH share 86% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTHLH/PTHrP Antibody (MAB6734)(1)

Publication using MAB6734

• L Zhang, J Qu, Y Qi, Y Duan, YW Huang, Z Zhou, P Li, J Yao, B Huang, S Zhang, D Yu EZH2 engages TGFbeta signaling to promote breast cancer bone metastasis via integrin beta1-FAK activation Nature Communications, 2022;13(1):2543. 2022 [PMID: 35538070] (IHC, Mouse)

Bioinformatics

• Gene Symbol: PTHLH
• Uniprot:
  • P12272()