SIX2 Antibody (3D7)

Images

 
Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody (M01), clone 3D7.Lane 1: SIX2 transfected lysate(32.3 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - NCAM1 expression in mouse embryonic kidney organ and hFK serum-free cultures. Mouse embryonic kidney organ culture stained for Ncam1, Six2 ...read more
Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 over-expressed 293 cell line, cotransfected with SIX2 Validated Chimera RNAi ( Cat # H00010736-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulation. Double labelling of sorted NCAM1+ cells for NCAM1 and SIX2: NCAM1 with DAPI (upper ...read more
ELISA: SIX2 Antibody (3D7) [H00010736-M01] - Detection limit for recombinant GST tagged SIX2 is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, RNAi, RNAi SP, S-ELISA
Clone
3D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SIX2 Antibody (3D7) Summary

Immunogen
SIX2 (AAH24033, 1 a.a. ~ 291 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSMLPTFGFTQEQVACVCEVLQQGGNIERLGRFLWSLPACEHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHAKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRSIWDGEETSYCFKEKSRSVLREWYAHNPYPSPREKRELTEATGLTTTQVSNWFKNRRQRDRAAEAKERENNENSNSNSHNPLNGSGKSVLGSSEDEKTPSGTPDHSSSSPALLLSPPPPGLPSLHSLGHPPGPSAVPVPVPGGGGADPLQHHHGLQDSILNPMSANLVDLGS
Specificity
SIX2 - sine oculis homeobox homolog 2 (Drosophila)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SIX2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • RNA Inhibition
  • RNAi sequence position
  • Sandwich ELISA
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA and RNAi Validation. Use in FLOW reported in scientific literature (PMID: 26295710). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23996934).
Publications
Read Publications using
H00010736-M01 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23996934). Rat reactivity reported in scientific literature (PMID: 26295710).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SIX2 Antibody (3D7)

  • homeobox protein SIX2
  • sine oculis homeobox (Drosophila) homolog 2
  • sine oculis homeobox homolog 2 (Drosophila)
  • Sine oculis homeobox homolog 2
  • SIX homeobox 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
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Publications for SIX2 Antibody (H00010736-M01)(8)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: FLOW, ICC/IF.


Filter By Application
FLOW
(1)
ICC/IF
(1)
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Filter By Species
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 8 of 8.
Publications using H00010736-M01 Applications Species
Reinke P and Kurtz A. Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells. Methods Mol Biol. 2019 [PMID: 30742266]
Monteiro Carvalho Mori da Cunha MG, Zia S, Oliveira Arcolino F et al. Amniotic Fluid Derived Stem Cells with a Renal Progenitor Phenotype Inhibit Interstitial Fibrosis in Renal Ischemia and Reperfusion Injury in Rats. PLoS One 2015 [PMID: 26295710] (FLOW, Rat) FLOW Rat
Mao Y, Francis-West P, Irvine KD. A Fat4-Dchs1 signal between stromal and cap mesenchyme cells influences nephrogenesis and ureteric bud branching. Development. 2015 Jun 26 [PMID: 26116666]
Wainwright EN, Wilhelm D, Combes AN et al. ROBO2 restricts the nephrogenic field and regulates Wolffian duct-nephrogenic cord separation. Dev Biol. 2015 Jun 23 [PMID: 26116176]
Hilliard Sylvia A, Yao Xiao, El-Dahr Samir S. Mdm2 is required for maintenance of the nephrogenic niche. Dev Biol. 2014 Mar 01 [PMID: 24440154] (Mouse) Mouse
Harari-Steinberg O, Metsuyanim S, Omer D et al. Identification of human nephron progenitors capable of generation of kidney structures and functional repair of chronic renal disease. EMBO Mol Med. 2013 Sep 2 [PMID: 23996934] (ICC/IF, Mouse) ICC/IF Mouse
Murphy AJ, Pierce J, de Caestecker C et al. SIX2 and CITED1, markers of nephronic progenitor self-renewal, remain active in primitive elements of Wilms' tumor. J Pediatr Surg. 2012 Jun 01 [PMID: 22703800]
Lee SH, Kim J, Ryu JY et al. Transcription coactivator Eya2 is a critical regulator of physiological hypertrophy. J Mol Cell Cardiol. 2011 Dec 14 [PMID: 22197309]

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Product General Protocols

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Bioinformatics

Gene Symbol SIX2
Entrez
OMIM
Uniprot