SIX1 Antibody (CL0185) - BSA Free

Western Blot: SIX1 Antibody (CL0185) [NBP2-52873] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATAD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: SIX1 Antibody (CL0185) [NBP2-52873] - Staining in RH30 cell line with Anti-SIX1 monoclonal antibody, showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.

Staining of human skeletal muscle shows strong nuclear positivity in myocytes.

Staining of human striated muscle shows strong nuclear immunoreactivity in the myocytes.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human salivary gland shows strong nuclear positivity in glandular cells.

Staining of human endocervix shows strong nuclear positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clone: CL0185
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SIX1 Antibody (CL0185) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from Q15475, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SIX1 Recombinant Protein Antigen (NBP2-52873PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SIX1 Antibody (CL0185) - BSA Free

• BOS3
• deafness, autosomal dominant 23
• DFNA23
• homeobox protein SIX1
• sine oculis homeobox (Drosophila) homolog 1
• sine oculis homeobox homolog 1 (Drosophila)
• Sine oculis homeobox homolog 1
• SIX homeobox 1
• TIP39

Background

The protein encoded by the SIX1 gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIX1 Antibody (NBP2-52873)(1)

Publication using NBP2-52873

• Jaime OG, Arias J, Pavani S et al. SIX1+PAX3+ identify a progenitor for myogenic lineage commitment from hPSCs Development (Cambridge, England) 2023-06-27 [PMID: 37366057] (IHC, ICC/IF, Human)

Bioinformatics

• Gene Symbol: SIX1