SIX1 Antibody (CL0185) - Azide and BSA Free

Staining in RH30 cell line with Anti-SIX1 monoclonal antibody) .

Analysis in human skeletal muscle and liver tissues using NBP3-43894 antibody. Corresponding SIX1 RNA-seq data are presented for the same tissues.

Staining of human endocervix shows strong nuclear positivity in glandular cells.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human skeletal muscle shows strong nuclear positivity in myocytes.

Staining of human salivary gland shows strong nuclear positivity in glandular cells.

Staining of human striated muscle shows strong nuclear immunoreactivity in the myocytes.

Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SIX1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0185
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

SIX1 Antibody (CL0185) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SIX1 Antibody (CL0185) - Azide and BSA Free (NBP2-52873) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q15475, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for SIX1 Antibody (CL0185) - Azide and BSA Free

• BOS3
• deafness, autosomal dominant 23
• DFNA23
• homeobox protein SIX1
• sine oculis homeobox (Drosophila) homolog 1
• sine oculis homeobox homolog 1 (Drosophila)
• Sine oculis homeobox homolog 1
• SIX homeobox 1
• TIP39

Background

The protein encoded by the SIX1 gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIX1