Sirtuin 3/SIRT3 Antibody

Western Blot: Sirtuin 3/SIRT3 Antibody [NB600-808] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Sirtuin 3/SIRT3 Antibody Summary

• Immunogen: Peptide with sequence C-QRETGKLDGPDK, from the C Terminus of the protein sequence according to NP_036371.1, NP_001017524.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIRT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx 28 kDa band observed in human liver lysates (calculated MW of 28.6 kDa according to NP_001017524.1).
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  Sirtuin 3/SIRT3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Sirtuin 3/SIRT3 Antibody

• EC 3.5.1
• EC 3.5.1.-
• hSIRT3
• mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
• NAD-dependent deacetylase sirtuin-3, mitochondrial
• SIR2L3
• SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
• sir2-like 3
• SIR2-like protein 3
• SIRT3
• sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
• Sirtuin 3
• sirtuin type 3

Background

This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sirtuin 3/SIRT3 Antibody (NB600-808)(2)

Publications using NB600-808

• Benavente CA, Schnell SA, Jacobson EL Effects of niacin restriction on sirtuin and PARP responses to photodamage in human skin. PLoS One. 2012-01-01 [PMID: 22860104] (WB, Human)
• Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial Sirtuin deacetylase, regulates mitochondrial function and thermogenesis in Brown adipocytes. J Biol Chem 2005-01-14 [PMID: 15653680]

Bioinformatics

• Gene Symbol: SIRT3
• Entrez:
  • 23410(Human)
• Uniprot:
  • NP_036371.1(Human)