SIRT6 Antibody - BSA Free

Western Blot: SIRT6 Antibody [NB100-2523] - Detection of SIRT6 in HeLa whole cell lysate.

Simple Western: SIRT6 Antibody [NB100-2523] - Simple Western lane view shows a specific band for SIRT6 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SIRT6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SIRT6 Antibody - BSA Free (NB100-2523) is a polyclonal antibody validated for use in WB and Simple Western. Anti-SIRT6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal region of the human SIRT6 protein (within residues 250-350). [Swiss-Prot Q8N6T7]
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIRT6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:10
  • Western Blot 0.5-2 ug/ml
• Application Notes: This SIRT6 antibody is useful for Western blot analysis, where a band is seen at approx. 39 kDa. A higher MW band approx. 59 kDa may be seen with lower dilutions (ie: 2 ug/ml). This antibody is not applicable for Immunocytochemistry / Immunofluorescence.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 49 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  SIRT6 Overexpression Lysate
  HeLa Whole Cell Lysate

• Control Peptide:

  SIRT6 Peptide (NB100-2523PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SIRT6 Antibody - BSA Free

• EC 3.5.1.-
• NAD-dependent deacetylase sirtuin-6
• SIR2L6sir2-related protein type 6
• SIR2-like protein 6
• sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
• sirtuin 6
• sirtuin type 6

Background

SIRT6 (SIR2-like protein 6) is a member of the yeast silent information regulator 2 (SIR2) family and possesses both strong ADP-ribosyltransferase activity as well as protein deacetylase activity which is dependent on NAD(+). SIRT6 exerts deacetylase activity towards histone H3K9Ac and H3K56Ac, and alters histone H3 acetylation in telomeric chromatin during cell cycle's S-phase. It deacetylates histone H3K9Ac at NFkB target promoters and downregulates a subset of NFkB target genes. Upon DNA damage, SIRT6 promotes DNA end resection via deacetylation of RBBP8. SIRT6 also serves as a corepressor of the transcription factor HIF1 alpha for regulating the expression of multiple glycolytic genes to control glucose homeostasis, and is required for normal IGF1 serum levels. SIRT6 play critical roles in maintaining telomere integrity, fine-tuning aging-associated gene expression programs, preventing genomic instability, and maintaining metabolic homeostasis, thus impacting on several pathways relevant for cancer, metabolism, aging, cellular senescence and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIRT6 Antibody (NB100-2523)(1)

Publication using NB100-2523

• Koczor CA, Saville KM, Andrews JF Et al. Temporal dynamics of base excision/single-strand break repair protein complex assembly/disassembly are modulated by the PARP/NAD+/SIRT6 axis Cell reports 2021-11-02 [PMID: 34731617] (WB, Human)

Bioinformatics

• Gene Symbol: SIRT6