SIRT6 Antibody

Images

 
Western Blot: SIRT6 Antibody [NB100-2523] - Detection of SIRT6 in HeLa whole cell lysate.
Simple Western: SIRT6 Antibody [NB100-2523] - Simple Western lane view shows a specific band for SIRT6 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

SIRT6 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human SIRT6 protein (within residues 250-350). [Swiss-Prot Q8N6T7]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIRT6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Simple Western 1:10
Application Notes
This SIRT6 antibody is useful for Western blot analysis, where a band is seen at approx. 39 kDa. A higher MW band approx. 59 kDa may be seen with lower dilutions (ie: 2 ug/ml). This antibody is not applicable for Immunocytochemistry / Immunofluorescence.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
SIRT6 Lysate (NBL1-15979)
HeLa Lysate (NB800-PC1)
Control Peptide
SIRT6 Peptide (NB100-2523PEP)

Reactivity Notes

Human. Immunogen sequence has 78% homology to mouse protein and 71% homology to rat.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SIRT6 Antibody

  • EC 3.5.1.-
  • NAD-dependent deacetylase sirtuin-6
  • SIR2L6sir2-related protein type 6
  • SIR2-like protein 6
  • sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
  • sirtuin 6
  • sirtuin type 6

Background

SIRT6 (SIR2-like protein 6) is a member of the yeast silent information regulator 2 (SIR2) family and possesses both strong ADP-ribosyltransferase activity as well as protein deacetylase activity which is dependent on NAD(+). SIRT6 exerts deacetylase activity towards histone H3K9Ac and H3K56Ac, and alters histone H3 acetylation in telomeric chromatin during cell cycle's S-phase. It deacetylates histone H3K9Ac at NFkB target promoters and downregulates a subset of NFkB target genes. Upon DNA damage, SIRT6 promotes DNA end resection via deacetylation of RBBP8. SIRT6 also serves as a corepressor of the transcription factor HIF1 alpha for regulating the expression of multiple glycolytic genes to control glucose homeostasis, and is required for normal IGF1 serum levels. SIRT6 play critical roles in maintaining telomere integrity, fine-tuning aging-associated gene expression programs, preventing genomic instability, and maintaining metabolic homeostasis, thus impacting on several pathways relevant for cancer, metabolism, aging, cellular senescence and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for SIRT6 Antibody (NB100-2523): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2523AF405
Alexa Fluor 488 Labeled NB100-2523AF488
Alexa Fluor 647 Labeled NB100-2523AF647
Alexa Fluor 700 Labeled NB100-2523AF700
Biotin Labeled NB100-2523B
DyLight 405 Labeled NB100-2523V
DyLight 488 Labeled NB100-2523G
DyLight 550 Labeled NB100-2523R
DyLight 650 Labeled NB100-2523C
HRP Labeled NB100-2523H

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Bioinformatics

Gene Symbol SIRT6