SIN3A Antibody

Western Blot: SIN3A Antibody [NB600-1263] - Analysis of K562 cell extract.

Immunocytochemistry/Immunofluorescence: SIN3A Antibody [NB600-1263] - Analysis of Sin3A (green) showing staining in the nucleus of A431 cells (right) compared to a negative control without primary antibody (left).

Immunohistochemistry-Paraffin: SIN3A Antibody [NB600-1263] - Analysis showing staining in the nucleus of human ovary carcinoma (right) compared to a negative control without primary antibody (left).

Chromatin Immunoprecipitation: SIN3A Antibody [NB600-1263] - Analysis of Sin3A performed using cross-linked chromatin from colon carcinoma cells treated with serum. Immunoprecipitation performed using a multiplex microplate Matrix ChIP assay with Sin3A polyclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of the Egr1 gene or exon-1 or exon-2 of Egr1. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. A representation of the Egr-1 locus is shown; boxes represent exons (black boxes = translated, white boxes = untranslated), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.

Immunocytochemistry/Immunofluorescence: SIN3A Antibody [NB600-1263] - Analysis of Sin3A (green) showing staining in the nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).

Immunocytochemistry/Immunofluorescence: SIN3A Antibody [NB600-1263] - Analysis of Sin3A (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SIN3A Antibody Summary

• Immunogen: Synthetic peptide corresponding to residues M(1) K R R L D D Q E S D V Y A A Q Q R R(19) of mouse mSin3A.
• Specificity: mSin 3A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIN3A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1 - 3 ul
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 0.5 ug/ml
• Application Notes: Use in FLOW reported in scientific literature (PMID 28260693).
• Theoretical MW: 145 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23280436).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SIN3A Antibody

• DKFZp434K2235
• FLJ90319
• Histone deacetylase complex subunit Sin3a
• KIAA0700
• paired amphipathic helix protein Sin3a
• SIN3 homolog A, transcription regulator (yeast)
• SIN3 homolog A, transcriptional regulator (yeast)
• SIN3A
• Transcriptional corepressor Sin3a
• transcriptional co-repressor Sin3A
• transcriptional regulator, SIN3A

Background

Mammalian Sin 3 (mSin 3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin 3A and mSin 3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin 3A and mSin 3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC 1, HDAC 2, RbAp 46, the methyl CpG binding protein MeCP 2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIN3A Antibody (NB600-1263)(6)

Publications using NB600-1263

• Dufour CR, Scholtes C, Yan M et al. The mTOR chromatin-bound interactome in prostate cancer Cell reports 2022-03-22 [PMID: 35320709] (ChIP, Human)
• Almenar-Queralt A, Merkurjev D et al. Chromatin establishes an immature version of neuronal protocadherin selection during the naive-to-primed conversion of pluripotent stem cells. Nat Genet 2019-01-12 [PMID: 31740836] (ChIP, Mouse)
• Srivas S, Thakur MK. Transcriptional co-repressor SIN3A silencing rescues decline in memory consolidation during scopolamine-induced amnesia. J Neurochem 2018-05-01 [PMID: 29494759] (Mouse)
• Siddiqui S, Lustig A, Carter A et al. Genomic deletion of GIT2 induces a premature age-related thymic dysfunction and systemic immune system disruption. Aging (Albany NY). 2017-03-04 [PMID: 28260693] (FLOW, Mouse)
• Rahmanto YS, Jung JG, Wu RC, Kobayashi Y. Inactivating ARID1A Tumor Suppressor Enhances TERT Transcription and Maintains Telomere Length in Cancer Cells. Journal of Biological Chemistry. 2016-03-07 [PMID: 26953344] (ChIP, Human)
• Feng D, Sangster-Guity N, Stone R et al. Differential requirement of histone acetylase and deacetylase activities for IRF5-mediated proinflammatory cytokine expression. J Immunol. 2010-11-15 [PMID: 20935208]

Bioinformatics

• Gene Symbol: SIN3A
• Entrez:
  • 25942(Human)
  • 20466(Mouse)
• Uniprot:
  • Q96ST3(Human)
  • Q60520(Mouse)