SIN3A Antibody

Images

 
Western Blot: SIN3A Antibody [NB600-1263] - Analysis of K562 cell extract.
Immunocytochemistry/ Immunofluorescence: SIN3A Antibody [NB600-1263] - Analysis of Sin3A (green) showing staining in the nucleus of A431 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: SIN3A Antibody [NB600-1263] - Analysis showing staining in the nucleus of human ovary carcinoma (right) compared to a negative control without primary antibody (left).
Chromatin Immunoprecipitation (ChIP): SIN3A Antibody [NB600-1263] - Analysis of Sin3A was performed using cross-linked chromatin from 1x10^6 HCT116 colon carcinoma cells treated with serum for 0, 15, and 30 minutes. ...read more
Immunocytochemistry/ Immunofluorescence: SIN3A Antibody [NB600-1263] - Analysis of Sin3A (green) showing staining in the nucleus of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: SIN3A Antibody [NB600-1263] - Analysis of Sin3A (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SIN3A Antibody Summary

Immunogen
Synthetic peptide corresponding to residues M(1) K R R L D D Q E S D V Y A A Q Q R R(19) of mouse mSin3A.
Specificity
mSin 3A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIN3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Chromatin Immunoprecipitation 1 - 3 ul
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:500
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Use in FLOW reported in scientific literature (PMID 28260693).
Theoretical MW
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB600-1263 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23280436).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SIN3A Antibody

  • DKFZp434K2235
  • FLJ90319
  • Histone deacetylase complex subunit Sin3a
  • KIAA0700
  • paired amphipathic helix protein Sin3a
  • SIN3 homolog A, transcription regulator (yeast)
  • SIN3 homolog A, transcriptional regulator (yeast)
  • SIN3A
  • Transcriptional corepressor Sin3a
  • transcriptional co-repressor Sin3A
  • transcriptional regulator, SIN3A

Background

Mammalian Sin 3 (mSin 3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin 3A and mSin 3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin 3A and mSin 3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC 1, HDAC 2, RbAp 46, the methyl CpG binding protein MeCP 2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SIN3A Antibody (NB600-1263)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for SIN3A Antibody (NB600-1263) (0)

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Secondary Antibodies

 

Isotype Controls

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SIN3A NB600-1263

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Bioinformatics

Gene Symbol SIN3A
Entrez
Uniprot