Western Blot: SIN3B Antibody [NBP2-20367] - Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE diluted at 1:500
Immunocytochemistry/ Immunofluorescence: SIN3B Antibody [NBP2-20367] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3B protein stained by SIN3B antibody [N1N2], N-term diluted at 1:500. Red: ...read more
Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367] - Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using antibody at 1:100 dilution.
Immunoprecipitation: SIN3B Antibody [NBP2-20367] - Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 ug of SIN3B antibody [N1N2], N-term Western blot analysis was performed using SIN3B ...read more
133 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP2-20367 in the following applications:
Mouse reactivity reported in scientific literature (PMID: 24691445).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Immunogen affinity purified
Alternate Names for SIN3B Antibody
Histone deacetylase complex subunit Sin3b
KIAA0700paired amphipathic helix protein Sin3b
SIN3 homolog B, transcription regulator (yeast)
SIN3 homolog B, transcriptional regulator (yeast)
SIN3 homolog B, transcriptional regulator
Transcriptional corepressor Sin3b
Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for SIN3B Antibody (NBP2-20367). (Showing 1 - 1 of 1 FAQ).
Does this antibody also react with mouse Sin3B?
NBP2-20367 has been validated in human for WB, ICC/IF, and IHC-P. The mouse SIN3B sequence has a 93% homology with the human sequence, and because of this, we do guarantee this antibody to perform in mouse for the validated applications listed on the datasheet. However, we have not yet tested this in mouse ourselves.
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