SIN3B Antibody

Images

 
Western Blot: SIN3B Antibody [NBP2-20367] - Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE diluted at 1:500
Immunocytochemistry/ Immunofluorescence: SIN3B Antibody [NBP2-20367] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3B protein stained by SIN3B antibody [N1N2], N-term diluted at 1:500. Red: ...read more
Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367] - Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using antibody at 1:100 dilution.
Immunoprecipitation: SIN3B Antibody [NBP2-20367] - Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 ug of SIN3B antibody [N1N2], N-term Western blot analysis was performed using SIN3B ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.96 mg/ml

Order Details

SIN3B Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SIN3B. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIN3B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-20367 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24691445).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.96 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SIN3B Antibody

  • Histone deacetylase complex subunit Sin3b
  • KIAA0700paired amphipathic helix protein Sin3b
  • SIN3 homolog B, transcription regulator (yeast)
  • SIN3 homolog B, transcriptional regulator (yeast)
  • SIN3 homolog B, transcriptional regulator
  • Transcriptional corepressor Sin3b

Background

Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SIN3B Antibody (NBP2-20367)(1)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
All Applications
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Human
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Mouse
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SIN3B Antibody (NBP2-20367). (Showing 1 - 1 of 1 FAQ).

  1. Does this antibody also react with mouse Sin3B?
    • NBP2-20367 has been validated in human for WB, ICC/IF, and IHC-P. The mouse SIN3B sequence has a 93% homology with the human sequence, and because of this, we do guarantee this antibody to perform in mouse for the validated applications listed on the datasheet. However, we have not yet tested this in mouse ourselves.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SIN3B
Uniprot