Sin1/MAPKAP1 Antibody - BSA Free

Western Blot: Sin1/MAPKAP1 Antibody [NB100-56482] - Analysis of Mip1 in human heart lysate in the 1) absence and 2) presence of immunizing peptide and in human skeletal muscle lysate in the 3) absence and 4) presence of immunizing peptide using this antibody. 5 ug/ml.

• Reactivity: Hu, Mu, Rt, Av, Bv, Ca, Ch, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Sin1/MAPKAP1 Antibody - BSA Free Summary

• Immunogen: A portion of amino acids 85-102 (NTAQRLERLRKERQNQIK) of human Mip1 was used as the immunogen.
• Specificity: There are multiple isoforms of this protein, ranging from approximately 310 amino acids to approximately 522 amino acids. Isoform distribution varies with tissue type.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPKAP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2 ug/ml
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Sin1/MAPKAP1 Antibody - BSA Free

• JC310
• MAPKAP1
• MEKK2-interacting protein 1
• MGC2745
• MIP1
• MIP1SIN1SIN1b
• Mitogen-activated protein kinase 2-associated protein 1
• mitogen-activated protein kinase associated protein 1
• mSIN1
• ras inhibitor MGC2745
• SAPK-interacting protein 1
• Sin1
• SIN1g
• stress-activated map kinase interacting protein 1
• Stress-activated map kinase-interacting protein 1
• stress-activated protein kinase-interacting 1
• target of rapamycin complex 2 subunit MAPKAP1
• TORC2 subunit MAPKAP1

Background

Mip1 is a novel member of a group of highly conserved proteins. Originally identified as a RAS suppressor protein, Mip1 is a human ortholog of S. cerevisiae Sin1. Studies reveal that Mip1 interacts with proteins involved in MAPK signaling including MEKK2 and participates in the regulation of MEKK2 activation and signaling. It also has been found to bind to JNK and might act as a scaffold molecule in the regulation of JNK signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPKAP1
• Entrez:
  • 79109(Human)
  • 227743(Mouse)
  • 296648(Rat)
• Uniprot:
  • Q92973(Human)