Serine Palmitoyltransferase 2 Antibody - BSA Free

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Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Western Blot Validation in Mouse 3T3/NIH Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Serine Palmitoyltransferase 2, NBP1-76573 (Lane ...read more
Immunocytochemistry/ Immunofluorescence: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Immunocytochemistry of SPT2 in 3T3 cells with SPT2 antibody at 10 ug/mL.
Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - 3T3 cell lysate with Serine Palmitoyltransferase 2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Independent Antibodies: Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Independent Antibody Validation via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. ...read more
Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Serine Palmitoyltransferase 2, NBP1-76573 (1 ug/mL), 1h ...read more
Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Western Blot Validation in Human Brain Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: Serine Palmitoyltransferase 2, NBP1-76573 (Lane ...read more
Immunocytochemistry/ Immunofluorescence: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Immunofluorescence of SPT2 in 3T3 cells with SPT2 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
 

Independent Antibodies

       

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Serine Palmitoyltransferase 2 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human Serine Palmitoyltransferase 2. The immunogen is located within the last 50 amino acids of Serine Palmitoyltransferase 2. Amino Acid Sequence: LDRPFDETTYEETED
Specificity
Serine Palmitoyltransferase 2 antibody is predicted to not cross-react with SPT1. Human Serine Palmitoyltransferase 2 has one isoform (562aa, 63kD). Mouse Serine Palmitoyltransferase 2 has one isoform (560aa, 63kD) and Rat Serine Palmitoyltransferase 2 also has one isoform (560aa, 63kD). NBP1-76573 can detect human, mouse and rat.
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPTLC2
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10-20 ug/ml
  • Immunofluorescence 20 ug/mL
  • Western Blot 0.5-1 ug/mL
Application Notes
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested. Predicted: 63kD; Observed: 63 kD
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Serine Palmitoyltransferase 2 Peptide (NBP1-76573PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Serine Palmitoyltransferase 2 Antibody - BSA Free

  • EC 2.3.1
  • EC 2.3.1.50
  • KIAA0526HSN1C
  • LCB 2
  • LCB2a
  • LCB2NSAN1C
  • Long chain base biosynthesis protein 2
  • Long chain base biosynthesis protein 2a
  • serine palmitoyltransferase 2
  • serine palmitoyltransferase, long chain base subunit 2
  • serine palmitoyltransferase, subunit II
  • Serine-palmitoyl-CoA transferase 2
  • SPT 2
  • SPT2

Background

Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT2 is the long chain base subunit 2 of mammalian serine palmitoyltransferase. SPT2 is catalytically active but needs its related protein SPT1 for its stabilization and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. As in the case with SPT1, mutations in the SPT2 gene can cause hereditary sensory and autonomic neuropathy type I (HSAN-I), resulting from a loss of SPT activity as well as the accumulation of the atypical and neurotoxic sphingoid metabolite 1-deoxy-sphinganine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SPTLC2