![Western Blot: Aquaporin-0 Antibody [NBP2-92773] - Analysis of extracts of various cell lines, using Aquaporin-0 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize2/Aquaporin-0 Antibody-Western Blot-NBP2-92773-img0004.jpg "Western Blot: Aquaporin-0 Antibody [NBP2-92773] - Analysis of extracts of various cell lines, using Aquaporin-0 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MIP (NP_036196.1). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MIP |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 28 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 50% glycerol, pH7.3. |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
PTMs for Aquaporin-0 Antibody (NBP2-92773)Learn more about PTMs related to Aquaporin-0 Antibody (NBP2-92773).
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| Gene Symbol | MIP |
![Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Paraffin-embedded rat brain using Aquaporin-0.](http://images.novusbio.com/fullsize2/Aquaporin-0-Antibody-Immunohistochemistry-Paraffin-NBP2-92773-img0003.jpg "Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Paraffin-embedded rat brain using Aquaporin-0.")
![Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Paraffin-embedded human stomach using Aquaporin-0.](http://images.novusbio.com/fullsize2/Aquaporin-0-Antibody-Immunohistochemistry-Paraffin-NBP2-92773-img0001.jpg "Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Paraffin-embedded human stomach using Aquaporin-0.")
![Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Paraffin-embedded mouse brain using Aquaporin-0.](http://images.novusbio.com/fullsize2/Aquaporin-0-Antibody-Immunohistochemistry-Paraffin-NBP2-92773-img0002.jpg "Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Paraffin-embedded mouse brain using Aquaporin-0.")
![Western Blot: CapG Antibody [NBP1-90214] - Analysis using Anti-CAPG antibody NBP1-90214 (A) shows similar pattern to independent antibody NBP1-90213 (B).](http://images.novusbio.com/images2/CapG-Antibody-Western-Blot-NBP1-90214-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CapG Antibody [NBP1-90214] - Staining of human cell line A-431 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/images2/CapG-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90214-img0008.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human lung, lymph node, skeletal muscle and small intestine using Anti-CAPG antibody (A) NBP1-90214 shows similar protein distribution across tissues to independent antibody NBP1-90215 (B).](http://images.novusbio.com/images2/CapG Antibody-Immunohistochemistry-Paraffin-NBP1-90214-img0021.jpg)
![Western Blot: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLN.](http://images.novusbio.com/images2/Neurolysin-Antibody-(OTI1D6)---Azide-and-BSA-Free-Western-Blot-NBP2-72961-img0002.jpg)
![Immunohistochemistry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NLN mouse monoclonal antibody.](http://images.novusbio.com/images2/Neurolysin-Antibody-(OTI1D6)---Azide-and-BSA-Free-Immunohistochemistry-NBP2-72961-img0007.jpg)
![Flow Cytometry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NLN.](http://images.novusbio.com/images2/Neurolysin-Antibody-(OTI1D6)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-72961-img0004.jpg)
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Pax8-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules-AQP1, distal tubules-THP and collecting ducts-AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Aquaporin-1-AQP1-Antibody-(1-A5F6)-Immunohistochemistry-NB600-749-img0002.jpg)
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localized to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/srep11061), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Aquaporin-1-AQP1-Antibody-(1-A5F6)-Immunohistochemistry-NB600-749-img0001.jpg)
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Slc22a6-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice (a); kidneys from vehicle treated mice were negative (b). Co-localisation studies revealed specific X-gal expression within proximal tubular epithelium (AQP1) with no expression detected in distal tubules (THP) or collecting ducts (AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Aquaporin 1-AQP1 Antibody (1-A5F6)-Immunohistochemistry-NB600-749-img0003.jpg)
![ELISA: Human PTH ELISA Kit (Colorimetric) [NBP2-60629]](http://images.novusbio.com/images2/Human PTH ELISA Kit (Colorimetric)-ELISA-NBP2-60629-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")