![Western Blot: Aquaporin-0 Antibody [NBP2-92773] - Analysis of extracts of various cell lines, using Aquaporin-0 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize2/Aquaporin-0-Antibody-Western-Blot-NBP2-92773-img0004.jpg "Western Blot: Aquaporin-0 Antibody [NBP2-92773] - Analysis of extracts of various cell lines, using Aquaporin-0 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MIP (NP_036196.1). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MIP |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 28 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 50% glycerol, pH7.3. |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Immunocytochemistry/Immunofluorescence: CapG Antibody [NBP1-90214] - Staining of human cell line A-431 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/images2/CapG-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90214-img0008.jpg)
![Western Blot: CapG Antibody [NBP1-90214] - Analysis using Anti-CAPG antibody NBP1-90214 (A) shows similar pattern to independent antibody NBP1-90213 (B).](http://images.novusbio.com/images2/CapG-Antibody-Western-Blot-NBP1-90214-img0010.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Analysis in human lung and liver tissues. Corresponding CAPG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90214-img0015.jpg)
![Western Blot: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NLN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/images2/Neurolysin-Antibody-(OTI1D6)---Azide-and-BSA-Free-Western-Blot-NBP2-72961-img0001.jpg)
![Western Blot: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLN.](http://images.novusbio.com/images2/Neurolysin-Antibody-(OTI1D6)---Azide-and-BSA-Free-Western-Blot-NBP2-72961-img0002.jpg)
![Flow Cytometry: Neurolysin Antibody (OTI1D6) - Azide and BSA Free [NBP2-72961] - Analysis of Jurkat cells, using anti-NLN antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/images2/Neurolysin-Antibody-(OTI1D6)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-72961-img0003.jpg)
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localized to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/doifinder/10.1038/srep11061), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Aquaporin-1-AQP1-Antibody-(1-A5F6)-Immunohistochemistry-NB600-749-img0001.jpg)
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Slc22a6-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice (a); kidneys from vehicle treated mice were negative (b). Co-localisation studies revealed specific X-gal expression within proximal tubular epithelium (AQP1) with no expression detected in distal tubules (THP) or collecting ducts (AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Aquaporin-1-AQP1-Antibody-(1-A5F6)-Immunohistochemistry-NB600-749-img0003.jpg)
![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Pax8-CreERT2 mediated beta-galactosidase expression. beta-galactosidase positivity was exclusively found in the tubular epithelium of the kidney of tamoxifen treated mice, with no staining observed in glomeruli or bloods vessels (a, framed area is shown enlarged in b). Kidneys from vehicle treated mice were negative (c). Co-localisation studies revealed X-gal expression within tubules of all renal tubular compartment (proximal tubules-AQP1, distal tubules-THP and collecting ducts-AQP2). Scale bars, 100um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0148055), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Aquaporin-1-AQP1-Antibody-(1-A5F6)-Immunohistochemistry-NB600-749-img0002.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MIP |
![Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Rat brain using MIP antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize2/Aquaporin-0-Antibody-Immunohistochemistry-Paraffin-NBP2-92773-img0003.jpg "Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Rat brain using MIP antibody at dilution of 1:100 (40x lens).")
![Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Human stomach using MIP antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize2/Aquaporin-0-Antibody-Immunohistochemistry-Paraffin-NBP2-92773-img0001.jpg "Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Human stomach using MIP antibody at dilution of 1:100 (40x lens).")
![Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Mouse brain using MIP antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize2/Aquaporin-0-Antibody-Immunohistochemistry-Paraffin-NBP2-92773-img0002.jpg "Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Mouse brain using MIP antibody at dilution of 1:100 (40x lens).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")