Siglec-H Antibody (23M14A10) - BSA Free

Western Blot: Siglec-H Antibody (23M14A10) [NBP2-27061] - WB analysis of mouse heart lysate using Siglec-H antibody (clone 23M14A10) at 3 ug/ml concentration.

Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) [NBP2-27061] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal mouse lung using 5 ug/ml concentration of SiglecH antibody (clone 23M14A10). Alkaline Phosphatase based detection was used for final staining development.

Flow Cytometry: Siglec-H Antibody (23M14A10) [NBP2-27061] - Analysis using the PE conjugate of NBP2-27061. Staining of freshly isolated Balb C mouse splenocytes with B220 (clone RA3-6B2) Alexa Fluor 488 antibody and with Rat IgG2a PE (isotype control, left) or anti-mouse Siglec H PE antibody (Clone: 23M14A10, right).

Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) [NBP2-27061] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal mouse kidney using 5 ug/ml concentration of SiglecH antibody (clone 23M14A10).The cells of various renal tubules showed specific immunopositivity for SiglecH.

Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) [NBP2-27061] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal mouse lung using 5 ug/ml concentration of SiglecH antibody (Clone 23M14A10). DAB based detection was used for final staining development.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: 23M14A10
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Siglec-H Antibody (23M14A10) - BSA Free Summary

• Immunogen: SkRSHTkRVERPATHMDQA (aa286-304)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: Siglech
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1ug/10^6cells~
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Western Blot 3-5ug/ml~
• Application Notes: In IHC-P application, 1M EDTA buffer pH 9.0 was used for antigen retrieval. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Siglec-H Antibody (23M14A10) - BSA Free

• 6430529G09Rik
• Siglech sialic acid binding Ig-like lectin H
• SiglecH
• Siglec-H

Background

Siglec-H, or sialic acid binding immunoglobulin-like lectin H, is a CD33 related protein expressed specifically by plasmacytoid dendritic cells or pDCs. Antigen-mediated delivery by Siglec-H in pDCs inhibits Th cell proliferation and further antibody responses. This leads to lessened expansion and Th1/Th17 polarization. Constant and low density antigen presentation by Siglec-H is thought to lead to an exhaustive type lessening of the response in CD4+ cells but not tolerance. A number of pathways have been proposed for the Siglec-H induced T cell hyporesponsiveness. Ever decreasing activation cycles in the presence of low level but continuous antigen delivery, as that observed with Siglec-H, have also been demonstrated to impart FoxP3+ Tregs immunosuppressive tolerogen-like effects. Ability to identify and control Siglec-H antigen mediated delivery activities with specific antibody provides a focal point for potential development of inflammation controls.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-H Antibody (NBP2-27061)(1)

Publication using NBP2-27061

• Saiga H, Ueno M, Tanaka T Et al. Transcription factor MafB-mediated inhibition of type I interferons in plasmacytoid dendritic cells International immunology 2021-11-03 [PMID: 34734243]

Bioinformatics

• Gene Symbol: Siglech