Siglec-7/CD328 Antibody

Western Blot: Siglec-7/CD328 Antibody [NBP2-20359] - Sample (50 ug of whole cell lysate) A: NCI-H929, B: IM9, C: U266, 10% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Siglec-7/CD328 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Siglec-7/CD328. The exact sequence is proprietary.
• Localization: Membrane; Single-pass type I membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIGLEC7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Siglec-7/CD328 Antibody

• Adhesion inhibitory receptor molecule 1
• adhesion inhibitory receptor molecule 1, siglec-7
• AIRM1
• AIRM-1
• AIRM1QA79 membrane protein
• CD328 antigen
• CD328
• CDw328
• D-siglec
• p75
• p75/AIRM1
• QA79
• sialic acid binding Ig-like lectin 7
• sialic acid binding immunoglobulin-like lectin 7
• sialic acid-binding Ig-like lectin 7
• Siglec7
• Siglec-7

Background

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SIGLEC7
• Uniprot:
  • Q9Y286()