Siglec-3/CD33 Antibody - BSA Free

Western Blot: Siglec-3/CD33 Antibody [NBP2-29619] - Analysis of CD33 in the partial recombinant protein.

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-29619] - Analysis of CD33 protein in a section of human small intestinal malignant stromal tumor using 5 ug/mL concentration of CD33 antibody. The cancer cells developed the expected membrane-cytoplasmic staining pattern.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Siglec-3/CD33 Antibody - BSA Free Summary

• Description: The canonical form of CD33 is 364 amino acids long (~39.8 kDa) and it is processed via cleavage of signal peptide (n-terminal amino acids 1-17). This protein is a single-pass type I membrane protein with an extracellular domain as well as a cytoplasmic tail, so that it is expected to produce a membrane-cytoplasmic pattern in immunostaining assays.
• Immunogen: Partial recombinant protein made to an internal portion of human CD33 protein (between residues 30-200) [UniProt P20138]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD33
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA reported in scientific literature (PMID 27535972)
  • Flow Cytometry reported in scientific literature (PMID 27535972)
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Siglec-3/CD33 Antibody - BSA Free

• CD33 antigen (gp67)
• CD33 antigen
• CD33 molecule
• CD33
• FLJ00391
• gp67
• myeloid cell surface antigen CD33
• p67
• sialic acid binding Ig-like lectin 3
• Sialic acid-binding Ig-like lectin 3
• Siglec3
• Siglec-3
• SIGLEC3gp67

Background

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-3/CD33 Antibody (NBP2-29619)(1)

Publication using NBP2-29619

• Hagemann UB, Wickstroem K, Wang E et al. In vitro and in vivo efficacy of a novel CD33 targeted thorium-227 conjugate for the treatment of acute myeloid leukemia. Mol. Cancer Ther. 2016-08-17 [PMID: 27535972] (ELISA, FLOW, Human)

Bioinformatics

• Gene Symbol: CD33
• Entrez:
  • 945(Human)
• Uniprot:
  • P20138()