Siglec-3/CD33 Antibody (HIM3-4) [Biotin]

Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) [Biotin] [NB500-509] - Surface staining of human peripheral blood leukocytes with anti-human CD33 (HIM3-4) biotin / streptavidin-APC.

• Reactivity: Hu, Pm
• Applications: Flow
• Clone: HIM3-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: 1.0 mg/ml

Siglec-3/CD33 Antibody (HIM3-4) [Biotin] Summary

• Additional Information: Clone HIM3-4 was used by HLDA to establish CD designation.
• Immunogen: NFMY-9s human cell line
• Localization: Cell Membrane
• Specificity: The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. HLDA V; WS Code M MA112 HLDA VI; WS Code M MA47
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD33
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-12 ug/ml
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Siglec-3/CD33 Antibody (HIM3-4) [Biotin]

• CD33 antigen (gp67)
• CD33 antigen
• CD33 molecule
• CD33
• FLJ00391
• gp67
• myeloid cell surface antigen CD33
• p67
• sialic acid binding Ig-like lectin 3
• Sialic acid-binding Ig-like lectin 3
• Siglec3
• Siglec-3
• SIGLEC3gp67

Background

CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD33