Siglec-3/CD33 Antibody (HIM3-4) [Janelia Fluor® 549]

• Reactivity: Hu, Pm
• Applications: Flow, CyTOF-ready
• Clone: HIM3-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 549

Siglec-3/CD33 Antibody (HIM3-4) [Janelia Fluor® 549] Summary

• Additional Information: Clone HIM3-4 was used by HLDA to establish CD designation.
• Immunogen: NFMY-9s human cell line
• Localization: Cell Membrane
• Specificity: The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. HLDA V; WS Code M MA112 HLDA VI; WS Code M MA47
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD33
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Siglec-3/CD33 Antibody (HIM3-4) [Janelia Fluor® 549]

• CD33 antigen (gp67)
• CD33 antigen
• CD33 molecule
• CD33
• FLJ00391
• gp67
• myeloid cell surface antigen CD33
• p67
• sialic acid binding Ig-like lectin 3
• Sialic acid-binding Ig-like lectin 3
• Siglec3
• Siglec-3
• SIGLEC3gp67

Background

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD33