![Western Blot: Siglec-15 Antibody [NBP3-06988] - Rat tissues. Loading: 15 ug of lysates per lane. Antibodies: (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize2/Siglec-15-Antibody-Western-Blot-NBP3-06988-img0004.jpg "Western Blot: Siglec-15 Antibody [NBP3-06988] - Rat tissues. Loading: 15 ug of lysates per lane. Antibodies: (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human SIGLEC15. The immunogen is located within amino acids 270-320 of SIGLEC15. |
| Specificity | Human SIGLEC15 has 2 isoforms, including isoform 1 (328aa, 36kD) and isoform 2 (174aa, 18kD). Mouse SIGLEC15 has one isoform (342aa, 37kD) and Rat SIGLEC15 also has one isoform (341aa, 37kD). 9017 can detect human and possibly mouse and rat isoforms. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SIGLEC15 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested. |
| Theoretical MW | 34-36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.](http://images.novusbio.com/images2/Siglec-1-CD169-Antibody-(HSn-7D2)-Immunohistochemistry-Frozen-NB600-534-img0003.jpg)
![Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/images2/Sialoadhesin-Antibody-(HSn-7D2)-Immunohistochemistry-Paraffin-NB600-534-img0001.jpg)
![Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled histogram), or Mouse IgG1 isotype control (MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).](http://images.novusbio.com/images2/Siglec-1-CD169-Antibody-(HSn-7D2)-Flow-Cytometry-NB600-534-img0002.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody at a dilution of 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/SYK-Antibody-Western-Blot-NBP1-32945-img0011.jpg)
![Western Blot: SYK Antibody [NBP1-32945] - SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody at a dilution of 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/SYK-Antibody-Western-Blot-NBP1-32945-img0012.jpg)
![Immunoprecipitation: SYK Antibody [NBP1-32945] - Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 ug of SYK antibody. Western blot analysis was performed using SYK antibody.](http://images.novusbio.com/images2/SYK-Antibody-Immunoprecipitation-NBP1-32945-img0013.jpg)
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Using the PE direct conjugate An intracellular stain was performed on K-562 cells with RANK/TNFRSF11A (9A725) antibody NB100-56029 (blue) and a matched isotype control NBP1-97005PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/images2/RANK-TNFRSF11A-Antibody-(9A725)-Flow-Cytometry-NB100-56508-img0009.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A [9A725] Antibody NB100-56508AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/images2/RANK-TNFRSF11A-Antibody-(9A725)-Flow-Cytometry-NB100-56508-img0010.jpg){kind=icon}
![Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508] - An intracellular stain was performed on MG-63 cells with RANK/TNFRSF11A Antibody (9A725) NB100-56508 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/images2/RANK-TNFRSF11A-Antibody-(9A725)---BSA-Free-Flow-Cytometry-NB100-56508-img0011.jpg){kind=icon}
![Immunohistochemistry-Paraffin: DAP10/HCST Antibody (982101) [Unconjugated] [MAB97861] - DAP10/HCST was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human DAP10/HCST Monoclonal Antibody (Catalog # MAB97861) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei.](http://images.novusbio.com/images2/DAP10-HCST-Antibody-(982101)-[Unconjugated]-Immunohistochemistry-Paraffin-MAB97861-img0001.jpg)
![Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Immortalized mouse podocytes. Image from verified customer review.](http://images.novusbio.com/images2/NFATC1-NFAT2-Antibody-Western-Blot-NB100-56732-img0007.jpg)
![Simple Western: NFATC1/NFAT2 Antibody [NB100-56732] - Lane view shows a specific band for NFAT2 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/NFATC1-NFAT2-Antibody-Simple-Western-NB100-56732-img0006.jpg)
![Western Blot: NFATC1/NFAT2 Antibody [NB100-56732] - Analysis of human (A)Ramos and (B) mouse RAW lysate using NFATc1 antibody at 1 ug/ml.](http://images.novusbio.com/images2/NFATC1-NFAT2-Antibody-Western-Blot-NB100-56732-img0002.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0014.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0015.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Siglec-15 Antibody (NBP3-06988)Discover more about diseases related to Siglec-15 Antibody (NBP3-06988).
| Pathways for Siglec-15 Antibody (NBP3-06988)View related products by pathway.
|
Research Areas for Siglec-15 Antibody (NBP3-06988)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SIGLEC15 |
![Western Blot: Siglec-15 Antibody [NBP3-06988] - Human and mouse lines. Loading: 15 ug of lysates per lane. Antibodies: (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize2/Siglec-15-Antibody-Western-Blot-NBP3-06988-img0001.jpg "Western Blot: Siglec-15 Antibody [NBP3-06988] - Human and mouse lines. Loading: 15 ug of lysates per lane. Antibodies: (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: Siglec-15 Antibody [NBP3-06988] - Human tissues. Loading: 15 ug of lysates per lane. Antibodies: (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize2/Siglec-15-Antibody-Western-Blot-NBP3-06988-img0002.jpg "Western Blot: Siglec-15 Antibody [NBP3-06988] - Human tissues. Loading: 15 ug of lysates per lane. Antibodies: (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: Siglec-15 Antibody [NBP3-06988] - Mouse tissues. Loading: 15 ug of lysates per lane. Antibodies: (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution](http://images.novusbio.com/fullsize2/Siglec-15-Antibody-Western-Blot-NBP3-06988-img0003.jpg "Western Blot: Siglec-15 Antibody [NBP3-06988] - Mouse tissues. Loading: 15 ug of lysates per lane. Antibodies: (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution")
![Immunohistochemistry-Paraffin: DAP12 Antibody [NBP1-85313] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/images2/DAP12-Antibody-Immunohistochemistry-Paraffin-NBP1-85313-img0005.jpg)
![Western Blot: DAP12 Antibody [NBP1-85313] - Analysis in control (vector only transfected HEK293T lysate) and TYROBP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/DAP12-Antibody-Western-Blot-NBP1-85313-img0008.jpg)
![Immunohistochemistry-Paraffin: DAP12 Antibody [NBP1-85313] - Analysis in human bone marrow and skeletal muscle tissues using NBP1-85313 antibody. Corresponding TYROBP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/DAP12-Antibody-Immunohistochemistry-Paraffin-NBP1-85313-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Staining in canine blood cells from a verified customer review.](http://images.novusbio.com/images2/Siglec-2-CD22-Antibody-(OTI1F12)-Immunocytochemistry-Immunofluorescence-NBP2-46125-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CD22.](http://images.novusbio.com/images2/Siglec-2-CD22-Antibody-(1F12)-Immunofluorescence-NBP2-46125-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) [NBP2-46125] - Siglec-2/CD22 Antibody (1F12) [NBP2-46125] - Analysis of HeLa cells.](http://images.novusbio.com/images2/Siglec-2-CD22-Antibody-(1F12)-Immunofluorescence-NBP2-46125-img0002.jpg)
![Sandwich ELISA: SCYL1 Antibody (2E5) [H00057410-M02] - Detection limit for recombinant GST tagged SCYL1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/images2/SCYL1-Antibody-(2E5)-Sandwich-ELISA-H00057410-M02-img0002.jpg)
![Western Blot: SCYL1 Antibody (2E5) [H00057410-M02] - SCYL1 monoclonal antibody (M02), clone 2E5. Analysis of SCYL1 expression in PC-12.](http://images.novusbio.com/images2/SCYL1-Antibody-(2E5)-Western-Blot-H00057410-M02-img0003.jpg)
![Western Blot: SCYL1 Antibody (2E5) [H00057410-M02] - SCYL1 monoclonal antibody (M02), clone 2E5. Analysis of SCYL1 expression in Hela S3 NE.](http://images.novusbio.com/images2/SCYL1-Antibody-(2E5)-Western-Blot-H00057410-M02-img0004.jpg)
![Immunohistochemistry: TDRD7 Antibody [NBP1-87315] - Staining of human testis shows cytoplasmic and nuclear positivity in subsets of testicular cells.](http://images.novusbio.com/images2/TDRD7-Antibody-Immunohistochemistry-NBP1-87315-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DAP1 Antibody [NBP2-92897] - Analysis of MCF-7 cells using DAP1 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/DAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92897-img0001.jpg)
![Western Blot: DAP1 Antibody [NBP2-92897] - Analysis of extracts of various cell lines, using DAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/images2/DAP1-Antibody-Western-Blot-NBP2-92897-img0003.jpg)
![Immunohistochemistry-Paraffin: DAP1 Antibody [NBP2-92897] - Rat kidney using DAP antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/images2/DAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-92897-img0002.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")