Siglec-5/Siglec-14 Antibody (194128)

Images

 
Human blood derived monocytes were stained with Mouse Anti-Human Siglec‑5/14 Monoclonal Antibody (Catalog # MAB10721, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by ...read more
Recombinant Human Siglec‑5 Fc Chimera (Catalog # 1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready, ELISA(Cap)
Clone
194128
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Siglec-5/Siglec-14 Antibody (194128) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec‑5/Siglec‑14
Lys18-Thr434
Accession # O15389
Specificity
Detects human Siglec‑5/Siglec‑14 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) Siglec-14 is observed and no cross-reactivity with rhSiglec-3, rhSiglec-7, or rhSiglec-9 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC5
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • Neutralization 0.04-0.2 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human Siglec-5/Siglec-14 Antibody (Catalog # MAB10721)
ELISA Detection: Human Siglec-5/Siglec-14 Biotinylated Antibody (Catalog # BAM10722)
Standard: Recombinant Human Siglec-5/CD170 Fc Chimera (Catalog # 1072-SL)
Publications
Read Publications using
MAB10721 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-5/Siglec-14 Antibody (194128)

  • Siglec-5/Siglec-14

Background

Siglecs (sialic acid binding Ig-like lectins) are a subgroup of the immunoglobulin superfamily that interact with sialic acids in glycoproteins and glycolipids. Siglec-5 binds to alpha-2, 3- and alpha-2, 6-linked sialic acid equally. It occurs as a disulfide-linked dimer of approximately 140 kDa with the highest expression levels in hematopoietic tissues. Siglec-5 has an inhibitory motif within its cytoplasmic domain. Siglec-14 is an activating receptor that shares 99.5% aa sequence identity with Siglec-5 through the first two extracellular Ig domains and displays a similar glycan binding preference.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-5/Siglec-14 Antibody (MAB10721)(6)

We have publications tested in 2 confirmed species: Human, Fungus.

We have publications tested in 4 applications: Flow Cytometry, ICC, Immunoprecipitation, Neutralization.


Filter By Application
Flow Cytometry
(2)
ICC
(1)
Immunoprecipitation
(1)
Neutralization
(2)
All Applications
Filter By Species
Human
(5)
Fungus
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB10721 Applications Species
R Suematsu, T Miyamoto, S Saijo, S Yamasaki, Y Tada, H Yoshida, Y Miyake Identification of lipophilic ligands of Siglec5 and 14 that modulate innate immune responses J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31551352] (Flow Cytometry, Fungus) Flow Cytometry Fungus
PJ Huang, PY Low, I Wang, SD Hsu, T Angata Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses J. Biol. Chem., 2018;0(0):. 2018 [PMID: 30377253] (Immunoprecipitation, Human) Immunoprecipitation Human
Izquierdo-Useros N, Lorizate M, Puertas M, Rodriguez-Plata M, Zangger N, Erikson E, Pino M, Erkizia I, Glass B, Clotet B, Keppler O, Telenti A, Krausslich H, Martinez-Picado J Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides. PLoS Biol, 2012;10(12):e1001448. 2012 [PMID: 23271952] (Neutralization, Human) Neutralization Human
Gunnarsson P, Fornander L, Pahlsson P, Grenegard M Sialic acid residues play a pivotal role in alpha(1)-acid glycoprotein (AGP)-induced generation of reactive oxygen species in chemotactic peptide pre-activated neutrophil granulocytes. Inflamm. Res., 2010;59(2):89-95. 2010 [PMID: 19669698] (Flow Cytometry, Human) Flow Cytometry Human
Carlin AF, Chang YC, Areschoug T, Lindahl G, Hurtado-Ziola N, King CC, Varki A, Nizet V Group B Streptococcus suppression of phagocyte functions by protein-mediated engagement of human Siglec-5. J. Exp. Med., 2009;206(8):1691-9. 2009 [PMID: 19596804] (Neutralization, Human) Neutralization Human
Angata T, Hayakawa T, Yamanaka M, Varki A, Nakamura M Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates. FASEB J., 2006;20(12):1964-73. 2006 [PMID: 17012248] (ICC, Human) ICC Human

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB10721U
Alexa Fluor 405 FAB10721V
Alexa Fluor 488 FAB10721G
Alexa Fluor 594 FAB10721T
Alexa Fluor 647 FAB10721R
Alexa Fluor 700 FAB10721N
Alexa Fluor 750 FAB10721S

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Bioinformatics

Gene Symbol SIGLEC5
Uniprot