Siglec-15 Antibody - BSA Free

Western Blot: Siglec-15 Antibody [NBP2-41162] - Analysis of SIGLEC15 in human kidney tissue lysate with SIGLEC15 antibody at (A) 1 and (B) 2 ug/mL.

Flow Cytometry: Siglec-15 Antibody [NBP2-41162] - Human macrophages, blood monocytes. Image from verified customer review.

Immunohistochemistry: Siglec-15 Antibody - BSA Free [NBP2-41162] - Immunohistochemistry of Siglec-15 in human kidney tissue with Siglec-15 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Siglec-15 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SIGLEC15. The immunogen is located within amino acids 50 - 100 of SIGLEC15. Amino Acid Squence: TFTHPHRHYDGPLT
• Specificity: SIGLEC15 antibody is predicted to not cross-react with other SIGLEC family members.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIGLEC15
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 - 2 ug/ml
• Application Notes: Siglec-15 antibody validated for FLOW from a verified customer review.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Siglec-15 Antibody - BSA Free

• CD33 antigen-like 3SIGLEC-15
• CD33 molecule-like 3
• CD33L3
• HsT1361
• sialic acid binding Ig-like lectin 15
• sialic acid-binding Ig-like lectin 15
• Siglec15
• Siglec-15

Background

SIGLEC15 binds sialylated glycoproteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-15 Antibody (NBP2-41162)(3)

Publications using NBP2-41162

• Zhan W, Bai F, Cai Y et al. Tumor stroma Siglec15 expression is a poor prognosis predictor in colon adenocarcinoma Journal of Cancer 2023-09-18 [PMID: 37859817] (IHC, Human)
  
  Details:
  1:500 dilution
• Lixuan Cui, Xiuyun Tian, Liang Yan, Xiaoya Guan, Bin Dong, Min Zhao, Ang Lv, Daoning Liu, Jianhui Wu, Chunyi Hao Expression and function of Siglec-15 in RLPS and its correlation with PD-L1: Bioinformatics Analysis and Clinicopathological Evidence International Journal of Medical Sciences 2022-01-01 [PMID: 36438917] (Flow Cytometry, Human)
• Lu Y, Li X, Xhang C et al. LINC00973 is involved in cancer immune suppression through positive regulation of Siglec 15 in clear cell renal cell carcinoma Cancer Sci 2020-08-31 [PMID: 32780490] (FLOW, Human)

Review for Siglec-15 Antibody (NBP2-41162) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 05/02/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Blood monocytes,Human macrophages
• Species: Human
• Lot: 6765-1702

Bioinformatics

• Gene Symbol: SIGLEC15