Shugoshin Antibody

Western Blot: Shugoshin Antibody [NBP2-20355] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Shugoshin Antibody [NBP2-20355] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Shugoshin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Shugoshin Antibody Summary

• Description: Novus Biologicals Rabbit Shugoshin Antibody (NBP2-20355) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Shugoshin. The exact sequence is proprietary.
• Localization: Nucleus; Centromere
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SGO1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Shugoshin Antibody

• FLJ14230
• hSgo1
• NY-BR-85
• Serologically defined breast cancer antigen NY-BR-85
• SGO
• Sgo1
• shugoshin 1AB protein
• shugoshin 1CD protein
• shugoshin 1EF protein
• shugoshin 1GH protein
• shugoshin 1KL protein
• shugoshin-like 1 (S. pombe)
• shugoshin-like 1

Background

Shugoshin (Sgo) proteins constitute a conserved protein family defined as centromeric protectors of Rec8-containing cohesin complexes in meiosis. It has been demonstrated that vertebrate shugoshin is required for preserving centromeric cohesion during mitosis. The presence of human Scc1 at centromeres in mitosis is dependent on human Sgo1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SGO1