INCENP Antibody (3D2)

Images

 
Western Blot: INCENP Antibody (3D2) [NBP2-37471] - Western blot analysis using INCENP mAb against HEK293 (1) and INCENP (AA: 369-583)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: INCENP Antibody (3D2) [NBP2-37471] - Immunofluorescence analysis of HepG2 cells using INCENP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: INCENP Antibody (3D2) [NBP2-37471] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using INCENP mouse mAb with DAB staining.
Flow Cytometry: INCENP Antibody (3D2) [NBP2-37471] - Flow cytometric analysis of Jurkat cells using INCENP mouse mAb (green) and negative control (red).
Western Blot: INCENP Antibody (3D2) [NBP2-37471] - Western blot analysis using INCENP mAb against human INCENP recombinant protein. (Expected MW is 50.2 kDa)
Immunohistochemistry: INCENP Antibody (3D2) [NBP2-37471] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using INCENP mouse mAb with DAB staining.
ELISA: INCENP Antibody (3D2) [NBP2-37471] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
3D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

INCENP Antibody (3D2) Summary

Immunogen
Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
INCENP
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • CyTOF-ready
Theoretical MW
105.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for INCENP Antibody (3D2)

  • binds and activates aurora-B and -C in vivo and in vitro
  • chromosomal passenger protein
  • FLJ31633
  • inner centromere protein antigens (135kD, 155kD)
  • inner centromere protein antigens 135/155kDa
  • inner centromere protein INCENP
  • inner centromere protein
  • MGC111393

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol INCENP