SHANK3 Antibody - BSA Free

Western Blot: SHANK3 Antibody [NBP2-41171] - Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry: SHANK3 Antibody - BSA Free [NBP2-41171] - Immunohistochemistry of SHANK3 in human brain tissue with SHANK3 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: SHANK3 Antibody - BSA Free [NBP2-41171] - Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SHANK3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SHANK3 Antibody - BSA Free (NBP2-41171) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-SHANK3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide near the center of human SHANK3. The immunogen is located within amino acids 890 - 940 of SHANK3. Amino Acid Squence: APRPPPAATPPERPKRRPR
• Specificity: At least three alternatively spliced transcript isoforms of SHANK3 are known to exist.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SHANK3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 2.5 ug/mL
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SHANK3 Antibody - BSA Free

• DEL22q13.3
• KIAA1650proline rich synapse associated protein 2
• Proline-rich synapse-associated protein 2
• prosap2
• PSAP2SH3 and multiple ankyrin repeat domains protein 3
• SH3 and multiple ankyrin repeat domains 3
• shank postsynaptic density protein
• Shank3
• SPANK-2

Background

The SHANK3 gene encodes a SH3 and multiple ankyrin repeat domain protein 3 that in isoform 1 is 1,741 amino acids long at 186 kDA and in isoform 2 is 1,732 amino acids long at 185 kDA. This protein functions as an adaptor in postsynaptic density of excitatory synapses. Through complexes that include GKAP/PSD-95 and Homer and the actin-based cytoskeleton, these excitatory synapses are connected with the postsynaptic membrane including NMDA-type and metabotropic glutamate receptor. Additionally, this protein may function in structural and organizational projects of the dendritic spine and the synaptic junction. SHANK3 gene participates in cytoplasmic microtubules, glutamatergic synapses, and signaling events regulated by ret tyrosine kinase. It is known to interact with genes PLCG1, FYN, SRC, ABL1, and PIK3R1. Defects in SHANK3 are associated with autism spectrum disorders and schizophrenia type 15. It is also associated with phelan-mcdermid syndrome, cerebritis, prostatitis, neuronitis, dementia, prostate cancer, hypogonadism, hepatitis a, jacobsen syndrome, and trisomy 22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SHANK3 Antibody (NBP2-41171)(1)

Publication using NBP2-41171

• Guerra M, Medici V, Weatheritt R et al. Fetal exposure to valproic acid dysregulates the expression of autism-linked genes in the developing cerebellum Translational psychiatry 2023-04-05 [PMID: 37019889] (WB, Mouse)

Bioinformatics

• Gene Symbol: SHANK3