SHANK1 Antibody - BSA Free

Immunohistochemistry: SHANK1 Antibody [NB300-167] - Synaptic distribution of Shank1/2 and somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. 5x magnification of hippocampus. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) and VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar =300 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.

Immunohistochemistry: SHANK1 Antibody [NB300-167] - Mouse retina using NB300-167.

Western Blot: SHANK1 Antibody [NB300-167] - Shank1a in rat brain lysate using NB300-167 at 1:500 dilution.

Immunohistochemistry: SHANK1 Antibody [NB300-167] - Synaptic distribution of Shank1/2 and somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) and VGLUT2 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3. Red arrow points toward the VGLUT2-band of the DG; scale bar = 300 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: SHANK1 Antibody [NB300-167] - An ASD biometal profile affects cell health and synapse numbers in vitro. Immunocytochemistry of hippocampal neurons DIV 14 grown under control, ASD1, and ASD2 conditions. The fluorescence intensity of Shank positive puncta was measured using antibodies specific for Shank1, Shank2, and Shank3. Exemplary images (upper panel) and quantification of average puncta signal intensity of 10 cells per condition (lower panel). Merged images show additional DAPI staining of the nucleus. Neurons grown under ASD1 conditions show a significant decrease of synaptic Shank proteins, while neurons under ASD2 conditions did not show a reduction. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/np/2015/985083/), licensed under a CC-BY license.

Immunohistochemistry: SHANK1 Antibody [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the intragranular mossy fibers and the stratum moleculare of the dentate gyrus. Confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1 (white; green in merge) and VGLUT1 (white; red in merge) antibodies. The upper rows (large squares) show the enlarged region (3 = SM; 2 = SG; 1 = PL; scale bar = 100 um), the bottom rows (small rectangles) show further enlargements (indicated in upper row by white rectangles) in the PL (1, top row) and SM (3, bottom row) (scale bar = 5 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: SHANK1 Antibody [NB300-167] - Colocalization of Shank immunoreactivity with VGLUT1 in the stratum radiatum/stratum lucidum of the CA3 region. confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1 (white; green in merge) and VGLUT1 (white;red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 um), the bottom row (small rectangles) shows a further enlargement (indicated in upper row by white rectangle) in the SR (scale bar = 5 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.

Immunohistochemistry: SHANK1 Antibody [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the stratum radiatum of the CA1. confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1 (white; green in merge) and VGLUT1 (white; red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 um), the bottom row (small rectangles) shows a further enlargement (indicated in upper row by white rectangle) in the SR (scale bar = 5 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.

Immunohistochemistry: SHANK1 Antibody [NB300-167] - Retinal tissue using NB300-167 at 1:800 dilution. Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SHANK1 Antibody - BSA Free Summary

• Immunogen: residues [SGPIYPGLFDIRSS] of the C-terminus of the SHANK1 protein.
• Specificity: Reacts with residues [SGPIYPGLFDIRSS] of the C-terminus of the SHANK1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SHANK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:800
  • Immunohistochemistry 1:250-1:750
  • Immunohistochemistry-Paraffin 1:250-1:750
  • Western Blot 1:500-1:1000
• Application Notes: This antibody is useful for Western blot, immunohistochemistry-Paraffin, and immunohistochemistry. The IF has been used at 1:800 on mouse G57B6

Reactivity Notes

Human, rat and mouse. Other species have not been tested.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SHANK1 Antibody - BSA Free

• SH3 and multiple ankyrin repeat domains 1
• SH3 and multiple ankyrin repeat domains protein 1
• Shank1
• Somatostatin receptor-interacting protein
• SSTR-interacting protein
• SSTRIPSPANK-1
• synamon

Background

Shank proteins are a family of scaffold proteins identified through their interaction with a variety of membrane and cyto-plasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are crucial in receptor tyrosine kinase signaling. Recent studies suggest that disruption of glutamate receptors at the Shank-postsynaptic platform could contribute to the destruction of post-synaptic density which underlies synaptic dysfunction and loss in Alzheimers disease. Shank1 might be relevant to human autism spectrum disorders due to its differential role in specific cognitive processes along with its importance in synapse structure and function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SHANK1 Antibody (NB300-167)(6)

Publications using NB300-167

• Baczyk M, Alami NO, Delestree N et al. Synaptic restoration by cAMP/PKA drives activity-dependent neuroprotection to motoneurons in ALS J. Exp. Med. 2020-08-03 [PMID: 32484501] (ICC/IF, Mouse)
• Wolstenholme JT, Drobna Z, Henriksen AD et al. Transgenerational Bisphenol A causes deficits in social recognition and alters post-synaptic density genes in mice Endocrinology 2019-06-12 [PMID: 31188430] (Mouse)
• Kerrisk Campbell M, Sheng M. USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-dependent Protein Levels J. Neurosci. 2018-05-07 [PMID: 29735556] (WB, Mouse)
• Heise C, Schroeder JC, Schoen M et al. Selective Localization of Shanks to VGLUT1-Positive Excitatory Synapses in the Mouse Hippocampus. Front Cell Neurosci. 2016-05-20 [PMID: 27199660] (ICC/IF, WB, Rat, Mouse)
• Hagmeyer S, Mangus K, Boeckers TM. Effects of Trace Metal Profiles Characteristic for Autism on Synapses in Cultured Neurons Neural Plasticity. 2015-02-19 [PMID: 25802764] (ICC/IF, Rat)
  
  Details:
  SHANK1 antibody was used for ICC-IF staining of Rat brain cultured hippocampal neurons grown under 3 different conditions (i) control cells/Ctrl grown in Neurobasal medium (ii) ASD1 cells grown in Neurobasal medium with addition of putative toxic metals 0.5 uM Cd, Cu, Hg, and 2uM Pb (iii) ASD2 cells grown in trace metal depleted Neurobasal medium that was reconstituted only for Mg and Ca, with addition of the putative toxic metals as mentioned for ASD1 conditions. For ICC-IF assay, the cells were - fixed in 4% PFA/1.5% sucrose/PBS at 4C for 20 minutes, permeablized 2X5 minutes with PBS having 0.2% Triton X-100 which followed blocking with 10% FBS/PBS for 1 hour at RT, incubated with primary antibody for 2 hours at RT followed by 1 hour incubation with Alexa conjugated secondary antibody and counterstained with DAPI (Figure 3).
• Schmeisser MJ, Ey E, Wegener S, Bockmann J et al. Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2. Nature 2012-01-01 [PMID: 22699619] (WB, Mouse)

Bioinformatics

• Gene Symbol: SHANK1
• Entrez:
  • 50944(Human)
  • 243961(Mouse)
  • 78957(Rat)