SHANK1 Antibody

Images

 
Western Blot: SHANK1 Antibody [NB300-167] - Shank1a in rat brain lysate using NB300-167 at 1:500 dilution.
Immunohistochemistry: SHANK1 Antibody [NB300-167] - Retinal tissue using NB300-167 at 1:800 dilution. Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack.
Immunohistochemistry: SHANK1 Antibody [NB300-167] - Mouse retina using NB300-167.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SHANK1 Antibody Summary

Immunogen
residues [SGPIYPGLFDIRSS] of the C-terminus of the SHANK1 protein.
Specificity
Reacts with residues [SGPIYPGLFDIRSS] of the C-terminus of the SHANK1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SHANK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:800
  • Immunohistochemistry 1:250-1:750
  • Immunohistochemistry-Paraffin 1:250-1:750
Application Notes
This antibody is useful for Western blot, immunohistochemistry-Paraffin, and immunohistochemistry. The IF has been used at 1:800 on mouse G57B6
Publications
Read Publications using
NB300-167 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Human, rat and mouse. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SHANK1 Antibody

  • SH3 and multiple ankyrin repeat domains 1
  • SH3 and multiple ankyrin repeat domains protein 1
  • Shank1
  • Somatostatin receptor-interacting protein
  • SSTR-interacting protein
  • SSTRIPSPANK-1
  • synamon

Background

Shank proteins are a family of scaffold proteins identified through their interaction with a variety of membrane and cyto-plasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are crucial in receptor tyrosine kinase signaling. Recent studies suggest that disruption of glutamate receptors at the Shank-postsynaptic platform could contribute to the destruction of post-synaptic density which underlies synaptic dysfunction and loss in Alzheimers disease. Shank1 might be relevant to human autism spectrum disorders due to its differential role in specific cognitive processes along with its importance in synapse structure and function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF7035
Species: Hu
Applications: IHC
NBP1-47610
Species: Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-15083
Species: Hu, Rt
Applications: WB, IHC, IHC-P
NBP1-84212
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB300-556
Species: Hu, Mu, Rt, In, Pm
Applications: WB, ChIP, B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-44999
Species: Hu, Mu
Applications: WB, ICC/IF
NBP2-37582
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC
NB300-556
Species: Hu, Mu, Rt, In, Pm
Applications: WB, ChIP, B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-00594
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NBP2-00810
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NBP2-15971
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85047
Species: Hu, Po
Applications: Simple Western, ICC/IF, IHC, IHC-P, ICC
AF8100
Species: Hu
Applications: WB, IHC
NBP1-85609
Species: Hu
Applications: IHC, IHC-P
AF2180
Species: Hu
Applications: WB, Simple Western, IP
NBP2-14907
Species: Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-21398
Species: Hu, Mu
Applications: WB, IHC-P, IP, IF

Publications for SHANK1 Antibody (NB300-167)(3)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(2)
WB
(2)
All Applications
Filter By Species
Mouse
(2)
Rat
(2)
All Species
Showing Publications 1 - 3 of 3.
Publications using NB300-167 Applications Species
Heise C, Schroeder JC, Schoen M et al. Selective Localization of Shanks to VGLUT1-Positive Excitatory Synapses in the Mouse Hippocampus. Front Cell Neurosci. May 20 2016 [PMID: 27199660] (ICC/IF, WB, Mouse, Rat) ICC/IF, WB Mouse, Rat
Hagmeyer S, Mangus K, Boeckers TM. Effects of Trace Metal Profiles Characteristic for Autism on Synapses in Cultured Neurons Neural Plasticity. 2015 Feb 19 [PMID: 25802764] (ICC/IF, Rat)

Details:
SHANK1 antibody was used for ICC-IF staining of Rat brain cultured hippocampal neurons grown under 3 different conditions (i) control cells/Ctrl grown in Neurobasal medium (ii) ASD1 cells grown in Neurobasal medium with addition of putative toxic metals 0.5 uM Cd, Cu, Hg, and 2uM Pb (iii) ASD2 cells grown in trace metal depleted Neurobasal medium that was reconstituted only for Mg and Ca, with addition of the putative toxic metals as mentioned for ASD1 conditions. For ICC-IF assay, the cells were - fixed in 4% PFA/1.5% sucrose/PBS at 4C for 20 minutes, permeablized 2X5 minutes with PBS having 0.2% Triton X-100 which followed blocking with 10% FBS/PBS for 1 hour at RT, incubated with primary antibody for 2 hours at RT followed by 1 hour incubation with Alexa conjugated secondary antibody and counterstained with DAPI (Figure 3).
ICC/IF Rat
Michael J Schmeisser, Elodie Ey, Stephanie Wegener, Juergen Bockmann, A Vanessa Stempel, Angelika Kuebler, Anna-Lena Janssen, Patrick T Udvardi, Ehab Shiban et al. Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2. Nature doi:10.1038/nature11015. 2012 [PMID: 22699619] (WB, Mouse) WB Mouse

Reviews for SHANK1 Antibody (NB300-167) (0)

There are no reviews for SHANK1 Antibody (NB300-167). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for SHANK1 Antibody (NB300-167): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SHANK1 Antibody (NB300-167) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SHANK1 Products

Bioinformatics Tool for SHANK1 Antibody (NB300-167)

Discover related pathways, diseases and genes to SHANK1 Antibody (NB300-167). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SHANK1 Antibody (NB300-167)

Discover more about diseases related to SHANK1 Antibody (NB300-167).
 

Pathways for SHANK1 Antibody (NB300-167)

View related products by pathway.

PTMs for SHANK1 Antibody (NB300-167)

Learn more about PTMs related to SHANK1 Antibody (NB300-167).
 

Research Areas for SHANK1 Antibody (NB300-167)

Find related products by research area.

Blogs on SHANK1

There are no specific blogs for SHANK1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SHANK1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SHANK1
Entrez