GKAP/DLGAP1 Antibody

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Western Blot: GKAP/DLGAP1 Antibody [NBP3-13181] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GKAP/DLGAP1 antibody (NBP3-13181) diluted at 1:3000.
Immunocytochemistry/ Immunofluorescence: GKAP/DLGAP1 Antibody [NBP3-13181] - GKAP/DLGAP1 antibody detects GKAP/DLGAP1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed ...read more
Immunohistochemistry-Frozen: GKAP/DLGAP1 Antibody [NBP3-13181] - GKAP/DLGAP1 antibody detects GKAP/DLGAP1 Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: ...read more
Western Blot: GKAP/DLGAP1 Antibody [NBP3-13181] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GKAP/DLGAP1 antibody (NBP3-13181) diluted at 1:3000.
Immunohistochemistry-Paraffin: GKAP/DLGAP1 Antibody [NBP3-13181] - GKAP/DLGAP1 antibody detects GKAP/DLGAP1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ...read more
Immunohistochemistry-Paraffin: GKAP/DLGAP1 Antibody [NBP3-13181] - GKAP/DLGAP1 antibody detects GKAP/DLGAP1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ...read more

Product Details

Summary
Reactivity Mu, Rt, Hu, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GKAP/DLGAP1 Antibody Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse GKAP/DLGAP1. The exact sequence is proprietary.
Predicted Species
Human (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLGAP1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for GKAP/DLGAP1 Antibody

  • DAP1
  • DAP-1-ALPHA
  • DAP-1DAP-1-BETA
  • discs, large (Drosophila) homolog-associated protein 1
  • disks large-associated protein 1
  • DLGAP1
  • GKAP
  • GKAPMGC88156
  • hGKAP
  • PSD-95/SAP90 binding protein 1
  • PSD-95/SAP90-binding protein 1
  • SAP90/PSD-95-associated protein 1
  • SAPAP1
  • SAPAP1Guanylate kinase-associated protein

Background

Members of the postsynaptic density-95 (PSD-95)/SAP90 family of membraneassociated guanylate kinase (MAGUK) proteins function as multimodular scaffolds that organize protein-signaling complexes at neuronal synapses. PSD-95/SAP90 binds guanylate kinase-associated protein (GKAP), also designated GK domain-binding protein, DAP-1-alpha, DAP-1-beta, PSD-95 binding protein, PSD-95/SAP90 associated protein, or SAPAP1, through the guanylate kinase domain. SAPAP1 is expressed widely in neurons of the cortex and hippocampus and in the Purkinje and granule cells of the cerebellum. N-terminal splice variants of SAPAP1 are expressed as 95 and 130 kDa proteins. SAPAP1 is localized specifically in the PSD of glutamatergic synapses, consistent with its direct interaction with PSD-95 family proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DLGAP1